The marginal Zone/Layer I as a novel niche for neurogenesis and gliogenesis in developing cerebral cortex
The marginal Zone/Layer I as a novel niche for neurogenesis and gliogenesis in developing cerebral cortex
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DOI：
10.1523/jneurosci.2418-07.2007
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发表时间：
2007-10-17
影响因子：
5.3
通讯作者：
Hedin-Pereira, Cecilia
中科院分区：
文献类型：
--
作者：
Costa, Marcos R.;Kessaris, Nicoletta;Hedin-Pereira, Cecilia
大脑皮层的细胞多样性被认为是由位于脑室区和脑室下区的祖细胞引起的。在这里，我们描述了一种新的祖细胞来源，位于小鼠大脑皮层的这两个主要发芽区之外，有助于神经发生和胶质瘤发生。增殖细胞首先出现在胚胎大脑皮层的前板中，在神经发生中后期边缘区进一步增加。胚胎边缘区祖细胞的分子特征以及从心室区和室下区分离的祖细胞克隆后代的大小和身份不同。延时视频显微镜和体外克隆分析显示，边缘区祖细胞池中含有大量少突胶质细胞或星形胶质细胞祖细胞，以及神经元和双能性祖细胞。因此，正如体内命运图谱所揭示的那样，边际区祖细胞在其命运特征以及起源区域（苍白区和苍白区下）方面都是异质的。由于层粘连蛋白γ 1-/-小鼠的基膜缺陷或Pax6小眼突变小鼠的边缘区细胞组成的改变都会导致边缘区祖细胞池的增加，因此边缘区的局部环境严格调节着这种新型祖细胞池的大小。总之，我们在发育中的大脑皮层边缘区发现了一种新的神经元和胶质祖细胞来源，其特性与脑室区和脑室下区祖细胞明显不同。
The cellular diversity of the cerebral cortex is thought to arise from progenitors located in the ventricular zone and subventricular zone in the telencephalon. Here we describe a novel source of progenitors located outside these two major germinative zones of the mouse cerebral cortex that contributes to neurogenesis and gliogenesis. Proliferating cells first appear in the preplate of the embryonic cerebral cortex and further increase in the marginal zone during mid and late neurogenesis. The embryonic marginal zone progenitors differ in their molecular characteristics as well as the size and identity of their clonal progeny from progenitors isolated from the ventricular zone and subventricular zone. Time-lapse video microscopy and clonal analysis in vitro revealed that the marginal zone progenitor pool contains a large fraction of oligodendrocyte or astrocyte progenitors, as well as neuronal and bipotent progenitors. Thus, marginal zone progenitors are heterogenous in regard to their fate specification, as well as in regard to their region of origin (pallial and subpallial) as revealed by in vivo fate mapping. The local environment in the marginal zone tightly regulates the size of this novel progenitor pool, because both basement membrane defects in laminin gamma 1-/- mice or alterations in the cellular composition of the marginal zone in Pax6 Small Eye mutant mice lead to an increase in the marginal zone progenitor pool. In conclusion, we have identified a novel source of neuronal and glial progenitors in the marginal zone of the developing cerebral cortex with properties notably distinct from those of ventricular zone and subventricular zone progenitors.