Fate mapping of neural crest cells during eye development using a Protein 0 promoter-driven transgenic technique
Fate mapping of neural crest cells during eye development using a Protein 0 promoter-driven transgenic technique
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使用蛋白质 0 启动子驱动的转基因技术绘制眼睛发育过程中神经嵴细胞的命运图谱
DOI：
10.1007/s00417-008-0845-0
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
4人2番目
中科院分区：
文献类型：
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作者：
Iwao K;Inatani M;4人2番目
目的绘制眼发育过程中神经嵴细胞的命运。方法使用表达Cre重组酶的转基因追踪发育中小鼠眼的神经嵴细胞，该重组酶由Protein 0启动子和aRosa 26 Cre响应报告基因控制，该报告基因在Cre介导的重组后产生β-半乳糖苷酶。结果胚胎第9.5天，在眼周节中检测到β-半乳糖苷酶阳性细胞。在E13.5-E18.5上，几种神经嵴细胞来源的组织，包括角膜基质、角膜内皮、虹膜角膜角、睫状体、原始玻璃体和眼睑被强烈染色。出生后的角膜基质中的染色下降，但坚持在推定的iridocorneal angle.ConclusionsProtein 0-Cre转基因小鼠提供了一个有条件的敲除策略，调查眼前段分化。
PurposeTo map neural crest cell fate during eye development.MethodsNeural crest cells were tracked in developing mouse eyes using a transgene expressing Cre recombinase controlled by theProtein 0promoter and aRosa26 Cre-responsive reporter gene that produced β-galactosidase after Cre-mediated recombination.Resultsβ-galactosidase-positive cells were detected in the periocular segment on embryonic day (E) 9.5. Several neural crest cell-derived tissues including corneal stroma, corneal endothelium, iridocorneal angle, ciliary body, primary vitreous and eyelid were strongly stained on E13.5–E18.5. The staining decreased in the corneal stroma after birth, but persisted in the presumptive iridocorneal angle.ConclusionsProtein 0-Cretransgenic mice offer a conditional knock-out strategy to investigate anterior eye segment differentiation.