An Optimized Protocol for Human Direct Cardiac Reprogramming
An Optimized Protocol for Human Direct Cardiac Reprogramming
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DOI：
10.1016/j.xpro.2019.100010
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发表时间：
2020-06-19
期刊：
影响因子：
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通讯作者：
Qian, Li
中科院分区：
文献类型：
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作者：
Garbutt, Tiffany A.;Zhou, Yang;Qian, Li
直接心脏重编程，将成纤维细胞转化为心肌细胞样细胞（iCMs），是一种治疗损伤心脏的有吸引力的方法。在这里，我们提出了一种新的人类心脏重编程方法，利用多顺反子三因子重编程鸡尾酒和一个microRNA。我们的方案产生心脏肌钙蛋白T阳性的人iCMs （hiCMs）的效率为40% -60%，大约是以前方案的两倍，仅需2周。由此产生的hicm显示心肌细胞样肌节结构、基因表达和钙振荡。有关本协议使用和执行的完整细节，请参阅Zhou et al.（2019）。
Direct cardiac reprogramming, the conversion of fibroblasts into cardiomyocytelike cells (iCMs), is an attractive approach to heal the injured heart. Here we present a new approach to human cardiac reprogramming that utilizes a polycistronic three-factor reprogramming cocktail and one microRNA. Our protocol produces cardiac Troponin T positive human iCMs (hiCMs) at an efficiency of 4 0%-60%, approximately double that of previous protocols, within just 2 weeks. The resulting hiCMs display cardiomyocyte-like sarcomere structure, gene expression, and calcium oscillation.For complete details on the use and execution of this protocol, please refer to Zhou et al. (2019).