Differential expression of atypical PKCs in the adult mouse brain
Differential expression of atypical PKCs in the adult mouse brain
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DOI：
10.1016/j.molbrainres.2004.05.009
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发表时间：
2004-08-23
期刊：
影响因子：
--
通讯作者：
Leitges, M
中科院分区：
文献类型：
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作者：
Oster, H;Eichele, G;Leitges, M
蛋白激酶C(PKC)家族是一类丝氨酸/苏氨酸激酶，在脑发育、突触可塑性、癫痫、脑缺血和神经细胞死亡等多种生理和病理生理过程中发挥重要作用。非典型PKCs亚群由三个成员组成，即PKCiota/lambda、PKcheeta和PKMzeta，氨基酸同源性很高。我们使用特定的RNA探针和原位杂交来确定所有三种异构体在成年小鼠脑中的表达模式。PKCiota和PKMzeta广泛表达于大脑皮层、边缘系统和丘脑。相反，PKC转录仅限于不同的前脑区域和小脑。在这里，我们首次对中枢神经系统中同型特异性的aPKC的分布进行了全面的概述，从而为进一步研究不同的aPKC在神经系统中的功能意义提供了坚实的基础。(C)2004爱思唯尔B.V.保留所有权利。
The protein kinase C (PKC) family of serine/threonine kinases plays a role in a variety of physiological and pathophysiological processes in the brain including development, synaptic plasticity, epilepsy, ischemia, and neuronal cell death. The subgroup of atypical PKCs (aPKCs) comprises of three members, PKCiota/lambda, PKCzeta, and PKMzeta, with high amino acid homology. We used specific RNA probes and in situ hybridization to determine the expression patterns of all the three isoforms in the adult mouse brain. PKCiota and PKMzeta were found to be broadly expressed in most of the cortex, the limbic system, and the thalamus. In contrast, PKC transcription was restricted to distinct forebrain areas and the cerebellum. Here we present a first comprehensive overview of isotype-specific aPKC distribution in the central nervous system, thereby providing a solid ground for further studies on the functional implications of the different aPKCs in the neuronal system. (C) 2004 Elsevier B.V. All rights reserved.