Effectiveness of recombinant protein AlnA in enhancing the extractability of polychlorinated biphenyls from contaminated soils.
Effectiveness of recombinant protein AlnA in enhancing the extractability of polychlorinated biphenyls from contaminated soils.
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DOI：
10.1016/j.jhazmat.2014.06.063
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发表时间：
2014-08
影响因子：
13.6
通讯作者：
Yan Wang;Cong Wang;Hejun Ren;Baolei Jia;Lan-ying Zhang
中科院分区：
文献类型：
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作者：
Yan Wang;Cong Wang;Hejun Ren;Baolei Jia;Lan-ying Zhang
多氯联苯（PCBs）的低水溶性和高吸附性是其生物修复的主要限制因素之一。在本研究中，首先研究了重组蛋白AlnA作为生物表面活性剂在提高多氯联苯可提取性方面的有效性。AlnA表面张力测量允许确定37.89 mN/m的最小值，临界胶束浓度为1.07 g/L。进行了多氯联苯的增溶和解吸实验。结果表明，AlnA在20 μ g/mL浓度下可使多氯联苯晶体的溶解度提高1.87~6.12倍，与吐温-80和环糊精相比，AlnA更容易从污染土壤中回收多氯联苯。盆栽试验表明，AlnA可使苜蓿根中PCBs的积累量增加1.4-2.1倍。同源模建和对接结果表明，多氯联苯同系物与AlnA的相互作用不同，氯代越高的多氯联苯与AlnA的结合亲和力越高。结果表明，AlnA在PCBs污染修复中具有很大的应用潜力。
One of the major limitations in bioremediation of polychlorinated biphenyls (PCBs) in contaminated soils is their low water solubility and high adsorbability. In the present study, effectiveness of recombinant protein AlnA as a biosurfactant in increasing extractability of PCBs was first investigated. The AlnA surface tension measurements allowed for determination of the minimum value of 37.89 mN/m with critical micelle concentration of 1.07 g/L. The solubilization of PCBs and desorption experiments were performed. It was observed that AlnA at 20 μg/mL could enhance solubility of selected crystal PCBs varying from 1.87 to 6.12 folds and it is easier to recover PCBs from contaminated soils compared with Tween-80 and cyclodextrin. Pot experiments indicated AlnA elevated 1.4–2.1-fold PCBs accumulation in roots by Alfalfa. The homology modeling and docking revealed PCBs congeners had different interactions with AlnA and higher-chlorinated PCBs possessed higher binding affinity. Results showed AlnA had considerable application potential in remediation of PCBs pollution.