Molecular mapping of a novel gene, Grh5, conferring resistance to green rice leafhopper (Nephotettix cincticeps Uhler) in rice, Oryza sativa L.
Molecular mapping of a novel gene, Grh5, conferring resistance to green rice leafhopper (Nephotettix cincticeps Uhler) in rice, Oryza sativa L.
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DOI：
10.1007/s00122-006-0270-x
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发表时间：
2006-08-01
影响因子：
5.4
通讯作者：
Yasui, Hideshi
中科院分区：
文献类型：
--
作者：
Fujita, Daisuke;Doi, Kazuyuki;Yasui, Hideshi
绿色稻叶蝉（Nephotettix cincticeps Uhler）是危害水稻的重要害虫之一。生活在东亚的温带地区。标题普通野生稻（Oryza rufipogon Griff）。（W1962），经抗生长激素释放激素（GRH）试验，发现该菌株对GRH具有高度抗性。为了了解GRH抗性的遗传基础，利用数量性状位点（QTL）分析了感病粳稻台中65（T65）与高抗品种W1962杂交的BC 1F 1群体。在水稻第8染色体上检测到一个GRH抗性主效QTL。然后通过标记辅助选择的高级回交开发了包含来自W1962的目标QTL区域片段的近等基因群体。进一步利用BC 4F 2群体进行分子定位，发现一个新的抗性基因位于水稻第8染色体长臂的远端区域，与简单重复序列标记RM 3754和RM 3761紧密连锁，命名为绿色稻叶蝉抗性5（Grh 5）。随后在作图群体的后代中开发了携带Grh 5的近等基因系（NIL）。将Grh 5-NIL的抗性水平与已开发的NIL的GRH抗性水平进行比较，发现Grh 5-NIL具有最高的抗性。发现的与Grh 5紧密连锁的DNA标记将有助于分子标记辅助选择，以改良水稻对GRH的抗性。
The green rice leafhopper (GRH), Nephotettix cincticeps Uhler, is one of the most serious insect pests affecting cultivated rice (Oryza sativa L.) in temperate regions of East Asia. An accession of the wild rice species, Oryza rufipogon Griff. (W1962), was found to be highly resistant to GRH by an antibiosis test. To understand the genetic basis of the GRH resistance, a BC1F1 population derived from a cross between a susceptible Japonica variety, Taichung 65 (T65), and a highly resistant accession W1962 was analyzed by quantitative trait loci (QTL) mapping. A single major QTL for GRH resistance was detected on rice chromosome 8. A nearly isogenic population containing segments of the targeted QTL region derived from W1962 was then developed through advanced backcrossing with marker-assisted selection. Further molecular mapping using a BC4F2 population revealed that a new resistance gene, designated as Green rice leafhopper resistance 5 (Grh5), was located on the distal region of the long arm of chromosome 8 and tightly linked to the simple sequence repeat markers RM3754 and RM3761. A nearly isogenic line (NIL) carrying Grh5 was subsequently developed in the progeny of the mapping population. The resistance level of Grh5-NIL was compared with those of developed NILs for GRH resistance and was found to have the highest resistance. The DNA markers found to be closely linked to Grh5 would be useful for marker-assisted selection for the improvement of resistance to GRH in rice.