Utility of the nuclear protein-coding gene, elongation factor-1 gamma (EF-1γ), for spider systematics, emphasizing family level relationships of tarantulas and their kin (Araneae: Mygalomorphae)
Utility of the nuclear protein-coding gene, elongation factor-1 gamma (EF-1γ), for spider systematics, emphasizing family level relationships of tarantulas and their kin (Araneae: Mygalomorphae)
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DOI：
10.1016/j.ympev.2006.07.018
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发表时间：
2007-02-01
影响因子：
4.1
通讯作者：
Hayashi, Cheryl Y.
中科院分区：
文献类型：
--
作者：
Ayoub, Nadia A.;Garb, Jessica E.;Hayashi, Cheryl Y.
蜘蛛系统学绝大多数依赖形态特征来解决更高层次的系统发育问题。对属水平以上蜘蛛的分子系统发育研究很少，部分原因是缺乏可用于扩增和测序的特征基因。在这里，我们展示了一种新的分子标记延伸因子-1 γ (EF-1 γ) 在识别蜘蛛下目 Mygalomorphae 的科级关系方面的系统发育实用性。我们包括来自 14 个科的 26 个 mygalomorph 属的基因组序列以及来自 Arancomorphae 下 10 个科的 cDNA 序列。我们发现了对 mygalomorphs 传统上分为非典型类（Antrodiaetidae、Atypidae 和 Mecicobothriidae）和非典型类（所有其他科）的强烈支持。一些具有多个属属代表的科被发现是多系或并系的，例如 Nemesiidae、Ctenizidae 和 Hexathelidae。可以从蜘蛛形动物扩增的基因组 EF-1 γ 的一小部分包含一个短内含子，这表明由于内含子的存在，无法扩增较长的基因组序列。该内含子可能对家族内蜘蛛形关系有用。使用 EF-1 gamma 的进化率提出了蜘蛛进化的暂定时间表，根据非参数平滑方法 (NPRS) 和化石约束估计每百万年成对分歧约为 0.22%。 (c) 2006 Elsevier Inc. 保留所有权利。
Spider systematics has overwhelmingly relied on morphological characters to resolve higher-level phylogenetic questions. Molecular phylogenetic studies of spiders above the genus level have been rare, partly because of a paucity of characterized genes available for amplification and sequencing. Here we show the phylogenetic utility of a new molecular marker, elongation factor-1 gamma (EF-1 gamma) for discerning family level relationships in the spider infraorder, Mygalomorphae. We included genomic sequences from 26 mygalomorph genera in 14 families as well as cDNA sequences from 10 families in the infraorder Arancomorphae. We found strong support for the traditional split of mygalomorphs into atypoids (Antrodiaetidae, Atypidae, and Mecicobothriidae) and non-atypoids (all other families). Some families with multiple generic representatives were found to be polyphyletic or paraphyletic, such as the Nemesiidae, Ctenizidae, and Hexathelidae. A small portion of genomic EF-1 gamma that could be amplified from araneomorphs contained a short intron, suggesting that longer genomic sequences could not be amplified due to the presence of introns. This intron may be useful for intra-familial araneomorph relationships. A tentative timeline for spider evolution is proposed using the evolutionary rate of EF-1 gamma, estimated to be approximately 0.22% pairwise divergence per million years based on a non-parametric smoothing method (NPRS) and fossil constraints. (c) 2006 Elsevier Inc. All rights reserved.