Modulation of serum and pituitary luteinizing hormone bioactivity by androgen in the rat.
Modulation of serum and pituitary luteinizing hormone bioactivity by androgen in the rat.
复制标题
雄激素对大鼠血清和垂体黄体生成素生物活性的调节。
作者：
A. Solano;A. García;K. Catt;M. Dufau
采用大鼠间质细胞睾酮测定法和完整大鼠LH （rLH）、rLH α和rLH β的RIAs法分析正常大鼠和去势大鼠血清和垂体LH的生物学和免疫学活性。去势后第5 ~ 25天血清和垂体LH的生物活性与免疫活性比均下降50%左右，60天恢复正常。去势雄性黄体生成素生物活性与免疫反应性比值的下降不是由于亚基产生的增加，而是由去势后给予丙酸睾酮（50微克/天）来预防的。这些观察结果表明，雄激素的代谢物改变了LH分子循环和储存形式的生物活性。
The biological and immunological activities of serum and pituitary LH were analyzed in normal and castrated rats using the rat interstitial cell testosterone assay and RIAs for intact rat LH (rLH), rLH alpha, and rLH beta. After castration, the bioactivity to immunoactivity ratio of both serum and pituitary LH decreased by about 50% between the 5th and 25th days and returned to normal by 60 days. This decrease in the bioactivity to immunoreactivity ratio of LH in castrated males was not due to increased subunit production and was prevented by the administration of testosterone propionate (50 micrograms/day) from the time of castration. These observations indicate that androgens of their metabolites modify the bioactivity of the circulating and stored forms of the LH molecule.