Preparation of bionanoreactor based on core-shell structured polyion complex micelles entrapping trypsin in the core cross-linked with glutaraldehyde
Preparation of bionanoreactor based on core-shell structured polyion complex micelles entrapping trypsin in the core cross-linked with glutaraldehyde
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DOI：
10.1021/bc034149m
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发表时间：
2004-03-01
影响因子：
4.7
通讯作者：
Kataoka, K
中科院分区：
文献类型：
--
作者：
Jaturanpinyo, M;Harada, A;Kataoka, K
近年来，聚离子复合物胶束被认为是一种很有前途的多肽和蛋白质的载体系统。然而，它的实用性受到其对环境的敏感性的限制，例如溶液的稀释和离子强度。在这项研究中，为了克服这些障碍，PIC胶束制备的阴离子嵌段共聚物，聚（乙二醇）-聚（α，β-天冬氨酸），和阳离子蛋白质，胰蛋白酶，交联与戊二醛通过席夫碱的形成。光散射技术的基础上，结果显示了一个有效的电阻的交联PIC胶束高盐浓度，这是一个关键参数控制的PIC胶束的结构。交联后胰蛋白酶的稳定性也得到显著提高。显然，作为一种生物活性剂和/或生物纳米储库，PIC胶束将蛋白质分子包埋在交联的核中，显示出改善的稳定性，使其在生物技术和制药科学领域中得到广泛应用。
Recently, the polyion complex (PIC) micelle has been suggested as a promising carrier system for peptide and proteins. However, its utilities are limited by its sensitivity to the environment such as dilution and ionic strength of the solution. In this study, to overcome these obstructions, PIC micelles prepared from an anionic block copolymer, poly(ethylene glycol)-poly(alpha,beta-aspartic acid), and a cationic protein, trypsin, were cross-linked with glutaraldehyde through the Schiff base formation. On the basis of a light scattering technique, the results revealed an efficient resistance of the cross-linked PIC micelle to a high salt concentration, which was a key parameter controlling the structure of the PIC micelles. Moreover, the stability of trypsin after cross-linking was remarkably improved. Evidently, as a bionanoreactor and/or bionanoreservoir, the PIC micelles entrapping protein molecules in the cross-linked core reveal an improved stability, allowing their wide application in the fields of biotechnology and pharmaceutical sciences.