Combinational Effect of Cell Adhesion Biomolecules and Their Immobilized Polymer Property to Enhance Cell-Selective Adhesion
Combinational Effect of Cell Adhesion Biomolecules and Their Immobilized Polymer Property to Enhance Cell-Selective Adhesion
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DOI：
10.1155/2016/2090985
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发表时间：
2016-01-01
影响因子：
3.3
通讯作者：
Kato, Ryuji
中科院分区：
文献类型：
--
作者：
Kurimoto, Rio;Kanie, Kei;Kato, Ryuji
虽然具有生物活性分子的医疗器械的表面固定是最广泛使用的改善生物相容性的策略之一，但生物材料的物理化学性质显著影响固定分子的活性。在这里，我们调查的组合效应的细胞选择性的生物分子和疏水性/亲水性的聚合物基板上的选择性粘附的内皮细胞（EC），成纤维细胞（FB），平滑肌细胞（SMC）。为了控制聚合物基底，将生物分子固定在温敏性聚（N-异丙基丙烯酰胺-co-2-羧基异丙基丙烯酰胺）（聚（NIPAAm-co-CIPAAm））接枝的玻璃表面上。通过改变生物分子固定化聚合物的分子构象，评价了其细胞选择性粘附性能。在RGDS（Arg-Gly-AspSer）肽固定化表面的情况下，所有细胞类型均粘附良好，而与表面疏水性无关。另一方面，当表面是亲水性的时，三精氨酸固定化的表面表现出FB选择性。此外，当表面是疏水性的时，三-Ile-固定化的表面表现出EC-选择性细胞粘附。我们相信，所提出的概念，这是用来研究生物分子固定化表面组合，是重要的，以产生新的生物材料，这是高度要求的医疗植入物和组织工程。
Although surface immobilization of medical devices with bioactive molecules is one of the most widely used strategies to improve biocompatibility, the physicochemical properties of the biomaterials significantly impact the activity of the immobilized molecules. Herein we investigate the combinational effects of cell-selective biomolecules and the hydrophobicity/ hydrophilicity of the polymeric substrate on selective adhesion of endothelial cells (ECs), fibroblasts (FBs), and smooth muscle cells (SMCs). To control the polymeric substrate, biomolecules are immobilized on thermoresponsive poly(N-isopropylacrylamide-co-2-carboxyisopropylacrylamide) (poly(NIPAAm-co-CIPAAm))-grafted glass surfaces. By switching the molecular conformation of the biomolecule-immobilized polymers, the cell-selective adhesion performances are evaluated. In case of RGDS (Arg-Gly-AspSer) peptide-immobilized surfaces, all cell types adhere well regardless of the surface hydrophobicity. On the other hand, a tri-Arg-immobilized surface exhibits FB-selectivity when the surface is hydrophilic. Additionally, a tri-Ile-immobilized surface exhibits EC-selective cell adhesion when the surface is hydrophobic. We believe that the proposed concept, which is used to investigate the biomolecule-immobilized surface combination, is important to produce new biomaterials, which are highly demanded for medical implants and tissue engineering.