Hormonal and developmental regulation of two arabinogalactan-proteins in xylem of loblolly pine (Pinus taeda)
Hormonal and developmental regulation of two arabinogalactan-proteins in xylem of loblolly pine (Pinus taeda)
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DOI：
10.1111/j.1399-3054.2000.1100415.x
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发表时间：
2000-12-01
影响因子：
6.4
通讯作者：
Loopstra, CA
中科院分区：
文献类型：
--
作者：
No, EG;Loopstra, CA
两种阿拉伯半乳聚糖蛋白的蛋白核心在火炬松木质部分化过程中以非常高的水平优先表达。阿拉伯半乳聚糖蛋白被认为在木质部发育中起重要作用。所描述的研究的目的是鉴定负责调控PtX3H6和PtX14A9表达的信号，并评估它们在幼苗发育期间的表达。应用植物激素生长素、赤霉素和乙烯的抑制剂对松树幼苗和随后的北方印迹分析表明，生长素和乙烯的抑制剂抑制了PtX 3H6的表达，所有3种激素抑制剂抑制了PtX 14 A9的表达。PtX3H6首先在萌发的胚胎中表达。直到几天后才观察到PtX14A9的表达。在幼茎中，这两个基因在径向扩展的下胚轴中比在伸长的上胚轴中表达更多，其中PtX14A9具有更高的下胚轴与上胚轴表达的比率。这些结果表明，PtX3H6和PtX14A9在幼苗发育过程中受到不同的调节，这些差异可能是由不同的激素信号介导的。
The protein cores of two arabinogalactan-proteins are preferentially expressed at very high levels in differentiating xylem of loblolly pine. Arabinogalactan-proteins are hypothesized to play important roles in xylem development, The objectives of the described research were to identify signals responsible for the regulation of PtX3H6 and PtX14A9 expression and to assess their expression during seedling development. Application of inhibitors of the phytohormones auxin, gibberellic acid, and ethylene to pine seedlings and subsequent northern blot analyses indicate that PtX3H6 expression is suppressed by inhibitors of auxin and ethylene and that PtX14A9 expression is suppressed by all 3 hormone inhibitors. PtX3H6 is first expressed in germinating embryos. Expression of PtX14A9 is not observed until several days later. In young stems, both genes are expressed more in the radially expanding hypocotyls than in elongating epicotyls with PtX14A9 having a higher ratio of hypocotyl to epicotyl expression. These results indicate that PtX3H6 and PtX14A9 are differently regulated during seedling development and that these differences may be mediated by different hormonal signaling.