Freeze-drying of red blood cells at ultra-Low temperatures.
Freeze-drying of red blood cells at ultra-Low temperatures.
复制标题
在超低温下冷冻干燥红细胞。
DOI：
10.1006/cryo.1998.2143
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发表时间：
1999
期刊：
影响因子：
2.7
通讯作者：
G. Rau
中科院分区：
文献类型：
--
作者：
V. Rindler;S. Lüneberger;P. Schwindke;I. Heschel;G. Rau
人红细胞（RBC）冷冻干燥和重悬后的溶血情况取决于真空干燥温度。在一项实验研究中，首先在羟乙基淀粉和麦芽糖存在下根据改良的高产冷冻保存方案将红细胞固化。然后，将它们在特殊的低温冷冻干燥装置中在-5至-65摄氏度之间的选定架子温度下进行真空干燥。随后，将干燥的样品重新悬浮在等渗的磷酸盐缓冲盐水溶液中。根据改良的盐水稳定性测试测定溶血。它随着搁板温度的降低而降低，直到 -35 摄氏度时达到最小值。进一步降低搁板温度不会产生任何有益效果；溶血甚至增加。为了解释这些结果，我们假设溶血取决于两种相反的破坏效应：（1）搁板温度越高，干燥过程中发生结构破坏的可能性就越大； (2)架子温度越低，水输送的驱动力越低；这可能导致细胞内脱水不完全，这意味着细胞在环境温度下不处于玻璃态。
The hemolysis of human red blood cells (RBCs) after freeze-drying and resuspension depends on the vacuum-drying temperature. In an experimental study, RBCs were first solidified based on a modified high-yield cryopreservation protocol in the presence of hydroxyethyl starch and maltose. Afterward, they were vacuum-dried in a special low-temperature freeze-drying device at selected shelf temperatures between -5 and -65 degrees C. Subsequently, the dried samples were resuspended in an isotonic, phosphate-buffered saline solution. The hemolysis was determined according to a modified saline stability test. It decreases with a decreasing shelf temperature until a minimum is reached at -35 degrees C. A further decrease of the shelf temperature has no beneficial effect; the hemolysis even increases. To interpret these results, we assume that the hemolysis depends on two contrary damaging effects: (1) the higher the shelf temperature, the higher the probability of structural damages occurring during drying; (2) the lower the shelf temperature, the lower the driving force for water transport; this may lead to an incomplete intracellular dehydration which means that the cells are not in a glassy state at ambient temperature.