Ethanol fixed brain imaging by phase-contrast X-ray technique. Journal of Physics
Ethanol fixed brain imaging by phase-contrast X-ray technique. Journal of Physics
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通过相差 X 射线技术乙醇固定脑成像。
DOI：
10.1088/1742-6596/425/2/022004
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
and Kazuhiro Ueda
中科院分区：
文献类型：
--
作者：
Tohoru Takeda;Thet-Thet-Lwin;Takuya Kunii;Ryota Sirai;Takahito Ohizumi;Hiroko Maruyama;Kazuyuki Hyodo;Akio Yoneyama;and Kazuhiro Ueda
利用X射线晶体干涉仪的双晶体位相衬度X射线成像技术可以描绘大鼠大脑皮质、白质和基底节等精细结构。在35keV的X射线能量下，乙醇固定脑的图像质量和对比度明显好于通常使用的福尔马林固定。乙醇固定的大脑皮质图像对比度是福尔马林固定的3倍以上。因此，乙醇固定技术可能更适合于35keV X射线能量下的脑结构细节成像。
The two-crystal phase-contrast X-ray imaging technique using an X-ray crystal interferometer can depict the fine structures of rat's brain such as cerebral cortex, white matter, and basal ganglia. Image quality and contrast by ethanol fixed brain showed significantly better than those by usually used formalin fixation at 35 keV X-ray energy. Image contrast of cortex by ethanol fixation was more than 3-times higher than that by formalin fixation. Thus, the technique of ethanol fixation might be better suited to image cerebral structural detail at 35 keV X-ray energy.