THE EFFECT OF CHEMICAL AND PHYSICAL AGENTS ON THE PHAGE RECEPTOR OF PHASE-II SHIGELLA-SONNEI
THE EFFECT OF CHEMICAL AND PHYSICAL AGENTS ON THE PHAGE RECEPTOR OF PHASE-II SHIGELLA-SONNEI
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DOI：
10.1084/jem.94.5.387
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发表时间：
1951-01-01
影响因子：
15.3
通讯作者：
GOEBEL, WF
中科院分区：
文献类型：
--
作者：
BARRY, GT;GOEBEL, WF
因为不同的预科生。从第二阶段S获得的体细胞抗原。宋内氏菌对T_3、T_4、T_7、大肠杆菌-痢疾杆菌的抑制能力各不相同。比较活细菌和死细菌上的病毒吸附率作为噬菌体受体破坏的标准。如果吸附速率相等，则假定微生物处理后受体未发生变化。所研究的杀菌剂包括苯酚、甲醛、环氧乙烷、乙醇、百里酚、高碘酸钠、氯仿、热、紫外线和紫外线。辐照、渗透冲击、声波振动和冻融。结果表明，当第二阶段S。用上述任何一种方法处理宋内氏藻，T7吸附到死生物体上的速率几乎没有改变，而T3的吸附速率则大大降低。在某些情况下，T4的吸附速率被抑制，但程度相对小于T3。只有轻微的照射的细菌与u。v.允许T3病毒以快速的速度吸附到死细胞上。在所有情况下，3种病毒以遵循T7>T4>T3的顺序的速率吸附到经处理的微生物上。一个陷阱。3种病毒在II期S的潜伏期。当营养肉汤用作培养基时，Sonnei给出的T3值为17 [±] 0.5 min，T4值为24.5 [±] 0.5 min，T7值为20 [±] 0.5 min。
Because different prepns. of the somatic antigen obtained from Phase II S. sonnei exhibit variations in their ability to inhibit T3, T4 and T7, coli-dysentery phages a study was made of the effects of physical and chemical agents on the phage-adsorption properties of intact bacterial cells. A comparison of the virus adsorption rates on live and dead bacteria served as the criterion for the destruction of phage receptors. If the adsorption rates were equal it was assumed that no alteration of the receptor occurred following treatment of the microorganisms. The bactericidal agents studied included phenol, formol, ethylene oxide, ethanol, thymol, Na periodate, chloroform, heat, u.-v. irradiation, osmotic shock, sonic vibration and freezing and thawing. It was found that when Phase II S. sonnei was treated by any of the above procedures the rate of adsorption of T7 to the dead organisms was little altered, whereas the rate of T3 adsorption was considerably reduced. The adsorption rate of T4 was in some cases depressed but to a relatively lesser degree than that of T3. Only mild irradiation of the bacteria with u.-v. permitted the T3 virus to adsorb to the dead cells at a rapid rate. In all cases the 3 viruses adsorbed to the treated microorganisms at rates which followed the order T7>T4>T3. A detn. of the latent periods of the 3 viruses in Phase II S. sonnei gave values of 17 [plus or minus] 0.5 min. for T3, 24.5 [plus or minus] 0.5 min. for T4 and 20 [plus or minus] 0.5 min. for T7 when nutrient broth served as the culture medium.