Detection of airborne rhinovirus and its relation to outdoor air supply in office environments
Detection of airborne rhinovirus and its relation to outdoor air supply in office environments
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DOI：
10.1164/rccm.200306-760oc
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发表时间：
2004-06-01
影响因子：
24.7
通讯作者：
Milton, DK
中科院分区：
文献类型：
--
作者：
Myatt, TA;Johnston, SL;Milton, DK
鼻病毒是呼吸道疾病患者发病的主要原因;然而，其传播方式存在争议。采用聚合酶链反应技术对办公室空气中鼻病毒进行检测，并与室外送风率进行相关检测。我们每个工作日从上午9点到下午5点进行空气采样，每次采样持续一个工作周。我们直接从滤膜上提取RNA进行鼻病毒的巢式逆转录-聚合酶链反应分析。还收集了患有上呼吸道感染的建筑物居住者的鼻灌洗样品。每10分钟记录一次室内二氧化碳（CO2）浓度，作为室外空气供应的替代品。为了增加CO2浓度的范围，我们每3个月调整一次室外空气供应率。广义加性模型表明，检测到空气中的鼻病毒和每周平均CO2浓度大于约100 ppm的概率之间的关联，控制协变量后。此外，从鼻灌洗液中获得的一种鼻病毒含有与同一周内收集的建筑物空气中的核酸序列相似的核酸序列。这些结果表明，在室外空气供应量低的建筑物中，居住者可能会增加暴露于来自建筑物居住者的传染性液滴核的风险。
Rhinoviruses are major causes of morbidity in patients with respiratory diseases; however, their modes of transmission are controversial. We investigated detection of airborne rhinovirus in office environments by polymerase chain reaction technology and related detection to outdoor air supply rates. We sampled air from 9 A.M. to 5 P.M. each workday, with each sample run for 1 work week. We directly extracted RNA from the filters for nested reverse transcriptase-polymerase chain reaction analysis of rhinovirus. Nasal lavage samples from building occupants with upper respiratory infections were also collected. Indoor carbon dioxide (CO2) concentrations were recorded every 10 minutes as a surrogate for outdoor air supply. To increase the range of CO2 concentrations, we adjusted the outdoor air supply rates every 3 months. Generalized additive models demonstrated an association between the probability of detecting airborne rhinovirus and a weekly average CO2 concentration greater than approximately 100 ppm, after controlling for covariates. In addition, one rhinovirus from a nasal lavage contained an identical nucleic acid sequence similar to that in the building air collected during the same week. These results suggest that occupants in buildings with low outdoor air supply may have an increased risk of exposure to infectious droplet nuclei emanating from a fellow building occupant.