Simulated Sunlight-Mediated Photodynamic Therapy for Melanoma Skin Cancer by Titanium-Dioxide-Nanoparticle-Gold-Nanocluster-Graphene Heterogeneous Nanocomposites
Simulated Sunlight-Mediated Photodynamic Therapy for Melanoma Skin Cancer by Titanium-Dioxide-Nanoparticle-Gold-Nanocluster-Graphene Heterogeneous Nanocomposites
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二氧化钛-纳米粒子-金-纳米团簇-石墨烯异质纳米复合材料模拟阳光介导的光动力疗法治疗黑色素瘤皮肤癌
DOI：
10.1002/smll.201603935
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发表时间：
2017-05-24
期刊：
影响因子：
13.3
通讯作者：
Zhang, Haiyuan
中科院分区：
文献类型：
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作者：
Cheng, Yan;Chang, Yun;Zhang, Haiyuan
模拟阳光有望作为一种光源，能够减轻患者在光动力治疗 (PDT) 期间的严重疼痛；然而，传统光敏剂对太阳光的利用效率较低，极大地限制了其应用。二氧化钛-纳米粒子-金-纳米簇-石墨烯（TAG）异质纳米复合材料旨在有效利用模拟阳光进行黑色素瘤皮肤癌PDT。金纳米团簇（Au NCs）的窄带隙以及Au NCs、二氧化钛纳米颗粒（TiO2 NPs）和石墨烯之间的交错能带可以有效利用模拟阳光和电子空穴对的分离，从而促进丰富的羟基和超氧自由基的产生。在模拟阳光照射下，TAG纳米复合材料可引发小鼠B16F1黑色素瘤细胞的一系列毒理学反应，如细胞内活性氧产生、谷胱甘肽消耗、血红素加氧酶-1表达和线粒体功能障碍，导致严重的细胞死亡。此外，在携带B16F1肿瘤异种移植物的小鼠中静脉内或瘤内施用生物相容性TAG纳米复合材料可以显着抑制肿瘤生长并引起严重的病理性肿瘤组织变化。所有这些结果都证明了显着的模拟阳光介导的 PDT 效果。
Simulated sunlight has promise as a light source able to alleviate the severe pain associated with patients during photodynamic therapy (PDT); however, low sunlight utilization efficiency of traditional photosensitizers dramatically limits its application. Titanium-dioxide-nanoparticle-gold-nanocluster-graphene (TAG) heterogeneous nanocomposites are designed to efficiently utilize simulated sunlight for melanoma skin cancer PDT. The narrow band gap in gold nanoclusters (Au NCs), and staggered energy bands between Au NCs, titanium dioxide nanoparticles (TiO2 NPs), and graphene can result in efficient utilization of simulated sunlight and separation of electron-hole pairs, facilitating the production of abundant hydroxyl and superoxide radicals. Under irradiation of simulated sunlight, TAG nanocomposites can trigger a series of toxicological responses in mouse B16F1 melanoma cells, such as intracellular reactive oxygen species production, glutathione depletion, heme oxygenase-1 expression, and mitochondrial dysfunctions, resulting in severe cell death. Furthermore, intravenous or intratumoral administration of biocompatible TAG nanocomposites in B16F1-tumor-xenograft-bearing mice can significantly inhibit tumor growth and cause severe pathological tumor tissue changes. All of these results demonstrate prominent simulated sunlight-mediated PDT effects.