Enhanced endothelialization and microvessel formation in polyester grafts seeded with CD34+ bone marrow cells
Enhanced endothelialization and microvessel formation in polyester grafts seeded with CD34+ bone marrow cells
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DOI：
10.1182/blood.v95.2.581
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发表时间：
2000-01-15
期刊：
影响因子：
20.3
通讯作者：
Wu, MHD
中科院分区：
文献类型：
--
作者：
Bhattacharya, V;McSweeney, PA;Wu, MHD
作者已经证明了自体骨髓预凝的聚对苯二甲酸乙二醇酯（PET）移植物加速内皮化。骨髓细胞有一个在其表面表达CD34抗原的早期祖细胞亚群。最近的一项体外研究表明，CD34（+）细胞可以分化为内皮细胞。目前的研究旨在确定CD34（+）祖细胞是否会促进犬模型中的血管移植愈合。作者将复合移植物植入狗的胸降主动脉（DTA） 4周。8毫米× 12厘米的复合移植物中心为4厘米的PET移植物，两端为4厘米的标准ePTFE移植物。整个复合材料涂有硅橡胶，使其不透水；因此，PET节段被保护，不受移植物周围和潘内生长的影响。研究移植物5个，对照移植物5个。手术前一天，抽取120 mt骨髓，利用免疫磁珠技术富集CD34（+）细胞，平均产生11.4 +/- 5.3 x 10(6)。在手术过程中，将这些细胞与静脉血混合，并将其植入复合研究移植物的PET段；对照移植物仅用静脉血处理。苏木精和伊红、免疫细胞化学和AgNO3染色显示，与对照组相比，种子移植物表面内皮化显著增加（92% +/- 3.4% vs 26.6% +/- 7.6%； P= 0.0001），新生内膜、移植物壁和外区微血管明显增加。在犬中，CD34（+）细胞播种促进血管移植内皮化；这提示了实际的治疗应用。
The authors have shown accelerated endothelialization on polyethylene terephthalate (PET) grafts preclotted with autologous bone marrow. Bone marrow cells have a subset of early progenitor cells that express the CD34 antigen on their surfaces. A recent in vitro study has shown that CD34(+) cells can differentiate into endothelial cells. The current study was designed to determine whether CD34(+) progenitor cells would enhance vascular graft healing in a canine model. The authors used composite grafts implanted in the dog's descending thoracic aorta (DTA) for 4 weeks. The 8-mm x 12-cm composite grafts had a 4-cm PET graft in the center and 4-cm standard ePTFE grafts at each end. The entire composite was coated with silicone rubber to make it impervious; thus, the PET segment was shielded from perigraft and pannus ingrowth. There were 5 study grafts and 5 control grafts. On the day before surgery, 120 mt bone marrow was aspirated, and CD34(+) cells were enriched using an immunomagnetic bead technique, yielding an average of 11.4 +/- 5.3 x 10(6). During surgery, these cells were mixed with venous blood and seeded onto the PET segment of composite study grafts; the control grafts were treated with venous blood only. Hematoxylin and eosin, immunocytochemical, and AgNO3 staining demonstrated significant increases of surface endothelialization on the seeded grafts (92% +/- 3.4% vs 26.6% +/- 7.6%; P=.0001) with markedly increased microvessels in the neointima, graft wall, and external area compared with controls. In dogs, CD34(+) cell seeding enhances vascular graft endothelialization; this suggests practical therapeutic applications.