An immunohistochemical study of the sciatic nerve in a rat knee immobilization model.
An immunohistochemical study of the sciatic nerve in a rat knee immobilization model.
复制标题
大鼠膝盖固定模型中坐骨神经的免疫组织化学研究。
DOI：
10.1589/jpts.28.1116
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发表时间：
2016-04
影响因子：
--
通讯作者：
Hoso M
中科院分区：
文献类型：
--
作者：
Yoshida S;Matsuzaki T;Hoso M
[目的]本研究通过免疫组织化学方法评估膝关节固定大鼠模型中坐骨神经周围的变化，并评估关节活动度训练的效果。 [对象与方法] 将21只雄性大鼠随机分为3组：对照组（C组）、固定组（I组）和运动组（E组）。 I组和E组大鼠右膝关节固定2周。在E组中，还进行了活动范围练习。实验结束后，对坐骨神经周围进行免疫组化观察。 [结果]免疫组化染色显示各组髓鞘及神经束膜均为层粘连蛋白阳性。在C组和E组中，所有大鼠均显示正常染色。相反，I组中有4只大鼠表现出弱标记。 [结论]我们的结果表明，固定在分子水平上改变了神经束膜，而运动范围的锻炼对于维持神经束膜的环境至关重要。
[Purpose] This study was performed to immunohistochemically evaluate changes in the periphery of the sciatic nerve in a rat model of knee immobilization, and to assess the effects of range of motion exercise. [Subjects and Methods] Twenty-one male rats were divided randomly into three groups: control (C), immobilized (I), and exercise (E group). Rats in the I and E groups had the right knee joint immobilized for 2 weeks. In the E group, range of motion exercise was also performed. After the experimental period, the periphery of the sciatic nerve was immunohistochemically observed. [Results] Immunohistochemical staining revealed that the myelin sheath and the perineurium in all groups were laminin positive. In the C and E groups, all rats showed normal staining. In contrast, 4 rats in the I group exhibited weak labeling. [Conclusion] Our results suggest that immobilization alters the perineurium at a molecular level and the range of motion exercise is essential for maintaining the environment of the perineurium.