Methods to Study RNA-Chromatin Interactions.
Methods to Study RNA-Chromatin Interactions.
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研究 RNA-染色质相互作用的方法。
DOI：
10.1007/978-1-0716-3191-1_20
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Chen,ZhenBouman
中科院分区：
文献类型：
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作者：
Sriram,Kiran;Luo,Yingjun;Malhi,NaseebK;Chen,AleyshaT;Chen,ZhenBouman
RNA在染色质的组织以及基因表达的调控中起着重要作用。虽然染色质是普遍附着的编码和非编码RNA，这些染色质相关的RNA（caRNA）的基因表达和细胞功能的影响和其潜在的机制刚刚开始解开。了解caRNA基因调控的潜在机制的一种方法是鉴定caRNA相关的基因组区域。几个研究小组已经开发了在一种RNA与整个基因组中捕获RNA-染色质相互作用的方法，即，“一对所有”或所有RNA相对于整个基因组，即，“全对全”的方式在本章中，我们将讨论几种最先进的方法，重点介绍它们背后的原理、实验步骤、优点和局限性以及它们的应用。我们的目标是为有兴趣使用这些技术探索caRNA的研究人员提供概述和指导。
RNA plays a fundamental role in the organization of chromatin as well as the regulation of gene expression. Although the chromatin is pervasively attached by both coding and noncoding RNAs, the impact of these chromatin-associated RNAs (caRNAs) on gene expression and cellular functions and their underlying mechanisms have just begun to be unraveled. One approach to understand the potential mechanism of gene regulation by caRNAs is to identify the caRNA-associated genomic regions. Several groups have developed methods to capture RNA–chromatin interactions in either one RNA vs the whole genome, i.e., “one-to-all” or all RNAs vs the whole genome, i.e., “all-to-all” manner. In this chapter, we discuss several state-of-the-art methods highlighting the principles behind them, the experimental procedures, the advantages and limitations, and their applications. Our goal is to provide an overview and guide to researchers interested in exploring caRNAs using these techniques.