Mulberry anthocyanins, cyanidin 3-rutinoside and cyanidin 3-glucoside, exhibited an inhibitory effect on the migration and invasion of a human lung cancer cell line
Mulberry anthocyanins, cyanidin 3-rutinoside and cyanidin 3-glucoside, exhibited an inhibitory effect on the migration and invasion of a human lung cancer cell line
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DOI：
10.1016/j.canlet.2005.04.033
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发表时间：
2006-04-28
期刊：
影响因子：
9.7
通讯作者：
Hsieh, Yih-Shou
中科院分区：
文献类型：
--
作者：
Chen, Pei-Ni;Chu, Shu-Chen;Hsieh, Yih-Shou
花青素作为天然着色剂存在于各种水果和蔬菜中，具有多种生物活性，并因其抗氧化特性而被广泛使用。此外，近年来的研究表明花青素具有多效性的抗癌和抗增殖作用。浆果提取物含有大量的花青素，通常用于饮食或一些治疗应用。在本研究中，我们首次观察到花青素- 3-芦丁苷和花青素- 3-葡萄糖苷（从Morus alba L.中提取）对高度转移的A549人肺癌细胞的迁移和侵袭具有剂量依赖性的抑制作用，但没有细胞毒性。结果表明，花青素- 3-葡萄糖苷和花青素- 3-芦丁苷处理可使基质matalloprotinase-2 （MMP-2）和尿激酶-溶酶原激活物（u-PA）的表达呈剂量依赖性降低，基质matalloprotinase-2组织抑制剂（TIMP-2）和溶酶原激活物抑制剂（PAI）的表达增强。进一步的半定量RT-PCR分析表明，这些变化都在转录水平上。此外，花青素- 3-芦丁苷和花青素- 3-葡萄糖苷处理还可以抑制c-Jun和NF-kappa b的激活，这些结果表明花青素可以降低癌细胞的体外侵袭性，因此可能在开发潜在的癌症治疗方法方面具有重要价值。2004爱思唯尔爱尔兰有限公司版权所有。
Anthocyanins, present in various fruits and vegetables as natural colorant, have been well characterized to be involved in various bioactive properties and are wildly used for their antioxidant properties. Furthermore, recent studies have revealed pleiotropic anticancer and antiproliferative capabilities of anthocyanin. Berry extract contains high amounts of anthocyanins and is commonly used in diet or in some therapeutic applications. In this study, we first observed that cyanidin 3-rutinoside and cyanidin 3-glucoside (extracted from Morus alba L.) exerted a dose-dependent inhibitory effect on the migration and invasion, of highly metastatic A549 human lung carcinoma cells in absence of cytotoxicity. The results showed that cyanidin 3-glucoside and cyanidin 3-rutinoside treatments could decrease the expressions of matrix matalloprotinase-2 (MMP-2) and urokinase-plasminogen activator (u-PA) in a dose-dependent manner and enhance the expression of tissue inhibitor of matrix matalloprotinase-2 (TIMP-2) and plasminogen activator inhibitor (PAI). Further analysis with semi-quantitative RT-PCR showed that these alterations were all on the transcriptional level. Further, a treatment of cyanidin 3-rutinoside and cyanidin 3-glucoside also resulted in an inhibition on the activation of c-Jun and NF-kappa B. Together, these result suggested that anthocyanins could decrease the in vitro invasiveness of cancer cells and therefore, may be of great value in developing a potential cancer therapy. (c) 2004 Elsevier Ireland Ltd. All rights reserved.