Functional characterization of a redundant plasmodium TRAP family invasin, TRAP-like protein, by aldolase binding and a genetic complementation test
Functional characterization of a redundant plasmodium TRAP family invasin, TRAP-like protein, by aldolase binding and a genetic complementation test
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DOI：
10.1128/ec.00089-08
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发表时间：
2008-06-01
期刊：
影响因子：
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通讯作者：
Matuschewski, Kai
中科院分区：
文献类型：
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作者：
Heiss, Kirsten;Nie, Hui;Matuschewski, Kai
有效和特异性的宿主细胞进入对于胞内病原体是极其重要的。顶复门的寄生虫是高度能动的，并积极进入宿主细胞。这些功能由TRAP家族的I型跨膜侵袭素介导，所述I型跨膜侵袭素将细胞外识别事件与寄生虫肌动蛋白-肌球蛋白运动机制连接。我们系统地测试了潜在的寄生虫侵袭素与肌动蛋白桥接分子醛缩酶的结合以及子孢子侵袭素TRAP的重要胞质结构域的互补。我们发现动合子侵袭素CTRP和一种新的，结构相关的蛋白质，称为TRAP样蛋白（TLP），是TRAP家族的功能成员。尽管TLP在侵袭性阶段表达，但靶向基因破坏在生命周期进展中显示出非重要作用。这是首次对疟疾寄生虫中编码冗余TRAP家族侵袭素的TLP进行遗传分析。
Efficient and specific host cell entry is of exquisite importance for intracellular pathogens. Parasites of the phylum Apicomplexa are highly motile and actively enter host cells. These functions are mediated by type I transmembrane invasins of the TRAP family that link an extracellular recognition event to the parasite actin-myosin motor machinery. We systematically tested potential parasite invasins for binding to the actin bridging molecule aldolase and complementation of the vital cytoplasmic domain of the sporozoite invasin TRAP. We show that the ookinete invasin CTRP and a novel, structurally related protein, termed TRAP-like protein (TLP), are functional members of the TRAP family. Although TLP is expressed in invasive stages, targeted gene disruption revealed a nonvital role during life cycle progression. This is the first genetic analysis of TLP, encoding a redundant TRAP family invasin, in the malaria parasite.