A new, fast and semi-automated size determination method (SASDM) for studying multicellular tumor spheroids
A new, fast and semi-automated size determination method (SASDM) for studying multicellular tumor spheroids
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DOI：
10.1186/1475-2867-5-32
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发表时间：
2005-01-01
影响因子：
5.8
通讯作者：
Bergstrom, Mats
中科院分区：
文献类型：
--
作者：
Monazzam, Azita;Razifar, Pasha;Bergstrom, Mats
背景资料：考虑到肿瘤学研究中使用多细胞肿瘤球体（MTS）的广泛性和重要性，通过准确和快速的方法确定MTS的大小是必不可少的。本研究建立了一种快速、有效的半自动化方法--SASDM法。将该方法应用于三种不同细胞系的MTS中并进行测试。结果：SASDM方法简便、快速、准确，适用于不同细胞系的MTS。结论：代谢综合征的代谢状态评估与图像分析相结合，为评价各种抗癌治疗的效果提供了一个很好的模型。
Background: Considering the width and importance of using Multicellular Tumor Spheroids (MTS) in oncology research, size determination of MTSs by an accurate and fast method is essential. In the present study an effective, fast and semi-automated method, SASDM, was developed to determinate the size of MTSs. The method was applied and tested in MTSs of three different cell-lines. Frozen section autoradiography and Hemotoxylin Eosin (H&E) staining was used for further confirmation.Results: SASDM was shown to be effective, user-friendly, and time efficient, and to be more precise than the traditional methods and it was applicable for MTSs of different cell-lines. Furthermore, the results of image analysis showed high correspondence to the results of autoradiography and staining.Conclusion: The combination of assessment of metabolic condition and image analysis in MTSs provides a good model to evaluate the effect of various anti-cancer treatments.