Relationship between interleukin-1 type 1 and 2 receptor gene polymorphisms and the expression level of membrane-bound receptors
Relationship between interleukin-1 type 1 and 2 receptor gene polymorphisms and the expression level of membrane-bound receptors
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DOI：
10.1038/cmi.2014.43
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发表时间：
2015-03-01
影响因子：
24.1
通讯作者：
Sennikov, S. V.
中科院分区：
文献类型：
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作者：
Vasilyev, F. F.;Silkov, A. N.;Sennikov, S. V.
多功能细胞因子白细胞介素-1（IL-1）的生物活性由其受体介导。本研究的目的是确定是否存在关联的单核苷酸多态性（SNP）在IL-1 1 1型和2型受体基因（IL 1 R1和IL 1 R2）和单核细胞亚群或可溶性IL-1受体的血清水平上的膜结合IL 1 R的表达水平。观察到在SNP rs 2234650：C>T中具有TT基因型的健康个体具有在其表面上表达IL 1 R1的完整CD 14（+）单核细胞的较低百分比。IL 1 R2中的SNP rs 4141134：T>C也与表达IL 1 R2的完整CD 3（+）T细胞的百分比相关。此外，在IL 1 R2中携带SNP rs 4141134：T>C的CC等位基因和SNP rs 2071008：T>G的TT等位基因的个体在脂多糖（LPS）刺激的PBMC培养物中的CD 14（+）单核细胞表面上具有较低的IL 1 R2密度。总之，本研究表明，IL-1受体基因多态性可能是影响免疫活性细胞膜结合IL-1受体（IL-1 R）表达的因素之一。
The biological activity of the multifunctional cytokine interleukin-1 (IL-1) is mediated by its receptors. The aim of this study was to determine if an association exists between single nucleotide polymorphisms (SNPs) in the IL-1 type 1 and 2 receptor genes (IL1R1 and IL1R2) and the expression level of membrane-bound IL1Rs on subpopulations of mononuclear cells or serum levels of soluble IL-1 receptors. It was observed that healthy individuals with the genotype TT in SNP rs2234650:C>T had a lower percentage of intact CD14(+) monocytes expressing IL1R1 on their surface. The SNP rs4141134:T>C in IL1R2 has also been associated with the percentage of intact CD3(+) T cells expressing IL1R2. Furthermore, individuals carrying the CC allele of SNP rs4141134:T>C and the TT allele of SNP rs2071008:T>G in IL1R2 had a lower density of IL1R2s on the surface of CD14(+) monocytes in lipopolysaccharide (LPS)-stimulated PBMC cultures. In summary, this study demonstrated that IL-1 receptor gene polymorphisms could be one of the factors influencing the expression of membrane-bound IL-1 receptors (IL1R) on immunocompetent cells.