An automated optofluidic biosensor platform combining interferometric sensors and injection moulded microfluidics
An automated optofluidic biosensor platform combining interferometric sensors and injection moulded microfluidics
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DOI：
10.1039/c7lc00524e
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发表时间：
2017-08-21
期刊：
影响因子：
6.1
通讯作者：
Lechuga, L. M.
中科院分区：
文献类型：
--
作者：
Szydzik, C.;Fernandez Gavela, A.;Lechuga, L. M.
阻碍光子生物传感器在护理点平台内实际实施的一个主要限制是它们与流体自动化子系统的集成。对于大多数诊断应用，光子生物传感器需要复杂的流体处理方案；这在竞争性免疫分析的情况下尤其突出，通常用于检测低浓度、低分子质量的生物标志物。为此，需要复杂的自动化微流控系统来实现光子生物传感器的全部护理点潜力。为了满足这一要求，我们提出了一种基于芯片阀门的微流控自动化模块，能够自动化这种复杂的流体处理。该模块是通过应用PDMS注塑制造技术实现的，该技术最近在我们之前的工作中描述，该技术能够实际制造常闭气动弹性体阀。在这项工作中，这些阀门被配置为实现芯片上隔膜泵的多路试剂寻址，提供循环竞争免疫分析自动化所需的样品和试剂处理能力。这项技术的应用简化了制造，并带来了大规模生产的潜力，将复杂的自动化微流体的点式集成带入了实用领域。该模块与高灵敏度、无标签的双模波导光子生物传感器集成在一起，并在概念验证生物传感试验的背景下进行了演示，检测低分子量抗生素四环素。
A primary limitation preventing practical implementation of photonic biosensors within point-of-care platforms is their integration with fluidic automation subsystems. For most diagnostic applications, photonic biosensors require complex fluid handling protocols; this is especially prominent in the case of competitive immunoassays, commonly used for detection of low-concentration, low-molecular weight biomarkers. For this reason, complex automated microfluidic systems are needed to realise the full point-of-care potential of photonic biosensors. To fulfil this requirement, we propose an on-chip valve-based microfluidic automation module, capable of automating such complex fluid handling. This module is realised through application of a PDMS injection moulding fabrication technique, recently described in our previous work, which enables practical fabrication of normally closed pneumatically actuated elastomeric valves. In this work, these valves are configured to achieve multiplexed reagent addressing for an on-chip diaphragm pump, providing the sample and reagent processing capabilities required for automation of cyclic competitive immunoassays. Application of this technique simplifies fabrication and introduces the potential for mass production, bringing point-of-care integration of complex automated microfluidics into the realm of practicality. This module is integrated with a highly sensitive, label-free bimodal waveguide photonic biosensor, and is demonstrated in the context of a proof-of-concept biosensing assay, detecting the low-molecular weight antibiotic tetracycline.