Expression of keratinocyte growth factor receptor correlates with expansive growth and early stage of gastric cancer.
Expression of keratinocyte growth factor receptor correlates with expansive growth and early stage of gastric cancer.
复制标题
DOI：
10.3892/ijo.28.2.307
复制
发表时间：
2006-02
影响因子：
5.2
通讯作者：
Tetsuro Matsunobu;T. Ishiwata;Masanori Yoshino;M. Watanabe;M. Kudo;Koshi Matsumoto;A. Tokunaga;T. Tajiri;Z. Naito
中科院分区：
文献类型：
--
作者：
Tetsuro Matsunobu;T. Ishiwata;Masanori Yoshino;M. Watanabe;M. Kudo;Koshi Matsumoto;A. Tokunaga;T. Tajiri;Z. Naito
角质形成细胞生长因子受体，也称为KGFR/FGFR 2 IIIb，主要位于上皮细胞中，并参与这些细胞的增殖。相反，最近的一项研究表明，KGFR在涎腺腺癌中的过表达诱导生长抑制、细胞分化和凋亡。我们试图阐明KGFR在正常和癌变的人胃组织和癌细胞系中的表达和作用。逆转录聚合酶链反应和蛋白质印迹分析显示KGFR mRNA及其蛋白在NUGC-4、KATO-III和MKN-7胃癌细胞系中表达，而在NS-8细胞系中不表达。免疫组化显示，正常胃上皮细胞腔面KGFR免疫反应性较弱。此外，KGFR免疫反应强烈检测到许多壁细胞的细胞核和细胞质。在胃癌组织中，KGFR表达于癌细胞的胞膜和胞浆中，占36.5%（46/126）。胃癌细胞KGFR表达与早期型大体表现、胃壁浸润浅、扩张生长型密切相关。KGFR表达与胃癌预后良好相关。这些结果表明，KGFR的表达在正常胃上皮细胞的分化和壁细胞功能中起重要作用。此外，胃癌细胞中KGFR表达水平降低或不表达可能与胃癌细胞的增殖和侵袭以及患者的不良预后相关。
The keratinocyte growth factor receptor, also known as KGFR/FGFR2 IIIb, is mainly localized in epithelial cells, and participates in the proliferation of these cells. In contrast, a recent study has revealed that the overexpression of KGFR in salivary adenocarcinoma induces growth inhibition, cell differentiation and apoptosis. We attempted to clarify the expression and role of KGFR in normal and cancerous human gastric tissues and cancer cell lines. Reverse-transcription polymerase chain reaction and Western blot analyses showed KGFR mRNA and its protein expression in NUGC-4, KATO-III and MKN-7 gastric cancer cell lines, but not in the NS-8 cell line. Immunohistochemically, KGFR immunoreactivity was weakly detected in the luminal surface of normal gastric epithelial cells. In addition, KGFR immunoreactivity was strongly detected in the nucleus and cytoplasm of many parietal cells. In gastric cancer tissue, KGFR was expressed in the cell membrane and cytoplasm of cancer cells in 46 of 126 (36.5%) cases. KGFR expression in gastric cancer cells was significantly associated with early-type macroscopic findings, shallow invasion of the gastric wall and expansive growth type. KGFR expression tended to correlate with a good prognosis in gastric cancer. These findings indicate that KGFR expression plays important roles in the differentiation of normal gastric epithelial cells and parietal cell functions. Furthermore, a decreased expression level or the non-expression of KGFR in gastric cancer cells may be associated with the proliferation and invasion of gastric cancer cells and a poor prognosis for the patient.