Molecular weight separation ofhot-water extractable soil organic matter using high-performance size exclusion chromatography with chemiluminescent nitrogen detection. Soil Sci
Molecular weight separation ofhot-water extractable soil organic matter using high-performance size exclusion chromatography with chemiluminescent nitrogen detection. Soil Sci
复制标题
使用高性能尺寸排阻色谱法和化学发光氮检测对热水可提取的土壤有机物进行分子量分离。
DOI：
10.1080/00380768.2011.565478
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
M.Moriizumi and T. Matsunaga
中科院分区：
文献类型：
--
作者：
E. Yamada;T. Hirota;N. Hatori;Y. Kitao;Y. Fuse;S. Aoki;H.Karatani;T. Matsunaga;M.Moriizumi and T. Matsunaga
为了表征土壤有机氮（N），我们应用高效体积排阻色谱（HPSEC）和荧光氮检测（CLND）对热水可提取有机质（HWEOM）的分子量进行了分离，这些有机质来自以2 kg m− 2 y − 1的速率施用或未施用植物残留堆肥的土壤，持续25年。N色谱图显示含N的HWEOM的分子量范围为0.1至100 kDa。未堆肥的土壤HWEOM的N色谱图在四种土壤（堆积型灰壤、低腐殖型灰壤、灰低地土和黄壤）中存在差异。在整个分子量范围内，施用堆肥的土壤中的氮素在HWEOM色谱图中的强度均大于未施用堆肥的土壤。这些结果表明，HPSEC/CLND是研究土壤水溶性有机氮结构和动态的有效工具。
To characterize soil organic nitrogen (N), we applied high-performance size exclusion chromatography (HPSEC) and chemiluminescent nitrogen detection (CLND) to the molecular weight separation of hot-water extractable organic matter (HWEOM) from soils that had been amended with or without plant residue compost applied at a rate of 2 kg m−2y−1for 25 years. The N chromatogram of the HWEOM showed that the HWEOM containing N had molecular weights ranging from 0.1 to 100 kDa. The N chromatograms of HWEOM from soils without compost differed among four soils (Cumulic Andosol, Low-humic Andosol, Gray Lowland soil, and Yellow soil). The N intensities in the HWEOM chromatograms were larger in the soils with compost amended than in those without compost over the whole range of molecular weights. These results demonstrate that HPSEC/CLND is a useful tool to investigate the structure and dynamics of water soluble organic N in soil.