BRUGIA-MALAYI - RAT-CELL INTERACTIONS WITH INFECTIVE LARVAE MEDIATED BY COMPLEMENT
BRUGIA-MALAYI - RAT-CELL INTERACTIONS WITH INFECTIVE LARVAE MEDIATED BY COMPLEMENT
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DOI：
10.1016/0014-4894(86)90043-3
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发表时间：
1986-12-01
影响因子：
2.1
通讯作者：
SUBRAHMANYAM, D
中科院分区：
文献类型：
--
作者：
CHANDRASHEKAR, R;RAO, UR;SUBRAHMANYAM, D
白化病大鼠巨噬细胞和中性粒细胞，在新鲜正常大鼠血清作为补体来源的存在下，粘附并促进体外杀死马来丝虫感染性幼虫。嗜酸性粒细胞本身在高细胞-靶比下具有轻微细胞毒性，但与巨噬细胞混合时可促进细胞毒性。嗜酸性粒细胞培养上清液增强巨噬细胞介导的对感染性幼虫的杀伤。新鲜正常大鼠血清中的补体通过交替途径起作用。用酵母聚糖A处理去除替代途径补体活性的新鲜正常大鼠血清，或在50 ℃加热20分钟去除因子B，或通过Sephadex G75柱去除因子D，均不能促进细胞粘附于寄生虫。免疫荧光法检测到感染幼虫表面有C3分子。马来丝虫感染性幼虫在新鲜正常大鼠血清中孵育时，补体有明显的消耗。白化病大鼠细胞在体外对感染性幼虫的细胞毒性比来自沙鼠或纳氏乳鼠的细胞更强，这可能反映了大鼠对B的更大抗性。马来传染病。有少得多的细胞浸润引进马来丝虫感染性幼虫到腹腔的沙鼠相比，大鼠和Mastomys natalensis表明更大的敏感性沙鼠腹腔内诱导感染。
Albino rat macrophages and neutrophils, in the presence of fresh normal rat serum as a source of complement, adhered to and promoted killing of Brugia malayi infective larvae in vitro. Eosinophils, by themselves, were marginally cytotoxic at a high cell-target ratio but promoted cytotoxicity when mixed with macrophages. Eosinophil culture supernatants enhanced the macrophage mediated killing of infective larvae. The complement of fresh normal rat serum was found to act by the alternate pathway. Fresh normal rat serum depleted of alternate pathway complement activity by treatment with zymosan A, or of Factor B by heating at 50 C for 20 min, or Factor D by passing through Sephadex G75 column, failed to promote cell adherence to the parasite. C3 molecules were detected on the surface of infective larvae by immunofluorescence. There was a significant consumption of complement when Brugia malayi infective larvae were incubated in fresh normal rat serum. Albino rat cells were more potent in inducing cytotoxicity to infective larvae in vitro than those from jird or Mastomys natalensis, which may reflect the greater resistance offered by the rat to B. malayi infection. There was much less cellular infiltration on introduction of Brugia malayi infective larvae into the peritoneal cavity of jirds compared to rats and Mastomys natalensis indicating the greater susceptibility of jirds to intraperitoneally induced infections.