Proteome analysis of Citrus sinensis L. (Osbeck) flesh at ripening time
Proteome analysis of Citrus sinensis L. (Osbeck) flesh at ripening time
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DOI：
10.1016/j.jprot.2009.09.005
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发表时间：
2009-11-02
影响因子：
3.3
通讯作者：
Foti, Salvatore
中科院分区：
文献类型：
--
作者：
Muccilli, Vera;Licciardello, Concetta;Foti, Salvatore
采用双向凝胶电泳和液相色谱-质谱联用技术对色素橙子（Citrus sinensis，cv.摩罗）与普通栽培品种（Cadenera）在成熟期的比较。比较摩罗和Cadenera的蛋白质图谱，发现有64个差异蛋白点。共鉴定出55个差异表达蛋白。蛋白质根据其推定的功能和已知的生物合成途径进行分类。大多数与糖代谢相关的蛋白质在摩罗中过表达，而与胁迫反应相关的蛋白质在Cadenera中过表达。属于未知/未命名和假设类的蛋白质的丰度可能与柑橘基因组上可用的不完整数据有关。次生代谢和氧化过程蛋白的相对丰度证实了花色素苷途径在摩罗中的关键作用，其特征在于成熟时的强烈着色。讨论了摩罗和Cadenera果实之间蛋白质差异表达的潜在作用，并将蛋白质组学结果与相同果实的已知转录本变异进行了比较。后者的分析突出了许多差异，确认有必要将蛋白质组学和转录组学方法相关联，以实现生物系统的更完整表征。(C)2009 Elsevier B. V.保留所有权利。
A combination of 2-DE and LC-MSMS approaches was used to identify the differentially expressed proteome of a pigmented sweet orange (Citrus sinensis, cv. Moro) in comparison with a common cultivar (Cadenera) at ripening time. The comparison of the protein patterns of Moro and Cadenera showed 64 differential expressed protein spots. Fifty-five differentially expressed proteins were identified. Proteins were classified according to their putative function and known biosynthetic pathways. Most of the proteins related to sugar metabolism were overexpressed in Moro, while those related to stress responses were overexpressed in Cadenera. The abundance of proteins belonging to Unknown/unnamed and Hypothetical classes could be associated to the incomplete data available on the Citrus genome. The relative abundance of Secondary metabolism and Oxidative process proteins substantiated the key role of the anthocyanin pathway in Moro, which is characterized by a strong pigmentation at ripening time. The potential role of protein differential expression between Moro and Cadenera fruits was discussed, and proteomic results were compared with the known variations of transcripts of the same fruits. The latter analyses highlighted many discrepancies, confirming the necessity to associate both proteomic and transcriptomic approaches in order to achieve a more complete characterization of the biological system. (C) 2009 Elsevier B.V. All rights reserved.