Identification and localization of dopamine receptor subtypes in rat olfactory mucosa and bulb: A combined in situ hybridization and ligand binding radioautographic approach
Identification and localization of dopamine receptor subtypes in rat olfactory mucosa and bulb: A combined in situ hybridization and ligand binding radioautographic approach
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DOI：
10.1016/s0891-0618(97)00215-9
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发表时间：
1997-05-01
影响因子：
2.8
通讯作者：
Moyse, E
中科院分区：
文献类型：
--
作者：
Coronas, V;Srivastava, LK;Moyse, E
哺乳动物的嗅球（OB）在肾小球层内含有大量的多巴胺能中间神经元。多巴胺已被证明可以在体内调节嗅觉信息处理的多个方面。这里使用目前可用的工具在 mRNA 和放射性配体结合位点水平上评估嗅球和粘膜的多巴胺受体。 D1A mRNA 在 OB 肾小球、丛状、二尖瓣细胞和颗粒层中发现，但在嗅粘膜中没有发现。嗅球和粘膜中不存在 D1B mRNA。使用两种不同的放射性配体在 OB 的肾小球层、丛状层、二尖瓣细胞层和颗粒层中检测到 D1 样结合位点，但在嗅粘膜中未检测到。因此，我们证明了先前怀疑的 OB 中是否存在 D1 样受体。 D2 mRNA 位于 OB 的肾小球层和颗粒层以及嗅粘膜中；在 OB 肾小球和颗粒层中发现了少量的 D3 mRNA，但在嗅粘膜中没有发现。在任一结构中均未检测到 D4 mRNA。在嗅粘膜固有层内发现了高密度的 D2 样、[I-125]碘磺必利标记的结合位点，并在 OB 的嗅神经层和肾小球层中得到了证实。在 OB 的嗅神经层和肾小球层中检测到微弱但显着密度的 [H-3]7-羟基-二丙基-氨基四氢萘 (OH-DPAT) 标记的 D3 结合位点，但在嗅粘膜中未检测到。 [I-125]碘磺必利与三种 D2/D3 选择性药物特异性结合的竞争产生了嗅球和粘膜中 D2 受体亚型的典型动力学。因此证明OB的嗅神经层和肾小球层含有主要的D2受体和少量的D3受体，而嗅粘膜仅表达D2受体。 (C) 1997 Elsevier Science B.V.
Olfactory bulb (OB) of mammals contains a large population of dopaminergic interneurons within the glomerular layer. Dopamine has been shown in vivo to modulate several aspects of olfactory information processing. The dopamine receptors of olfactory bulb and mucosa are assessed here at the levels of mRNAs and radioligand binding sites with presently available tools. D1A mRNA was found in OB glomerular-, plexiform-, mitral-cell and granular layers, but not in olfactory mucosa. D1B mRNA was absent in olfactory bulb and mucosa. D1-like binding sites were detected with two distinct radioligands, in glomerular-, plexiform-, mitral cell- and granular layers of OB but not in olfactory mucosa. We thus demonstrate the previously doubtful presence of D1-like receptors in OB. D2 mRNAs were localized in the glomerular and granular layers of OB and in olfactory mucosa; lesser amounts of D3 mRNAs were found in OB glomerular and granular layer, but not in olfactory mucosa. No D4 mRNA was detected in either structure. High densities of D2-like, [I-125]Iodosulpride-labelled binding sites, were revealed within lamina propria of olfactory mucosa, and confirmed in the olfactory nerve- and glomerular layers of OB. A faint but significant density of [H-3]7-hydroxy-dipropyl-aminotetralin (OH-DPAT) labelled, D3 binding sites was detected in olfactory nerve- and glomerular layers of OB, but not in olfactory mucosa. Competition of [I-125]Iodosulpride specific binding by three D2/D3 selective drugs yielded kinetics typical of the D2 receptor subtype in olfactory bulb and mucosa. Olfactory nerve- and glomerular layers of OB are proved thus to contain a predominant contingent of D2 receptors and a minor population of D3 receptors, while olfactory mucosa expresses only D2 receptors. (C) 1997 Elsevier Science B.V.