Separation of furostanol saponins by supercritical fluid chromatography
Separation of furostanol saponins by supercritical fluid chromatography
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超临界流体色谱分离呋甾烷皂苷
DOI：
10.1016/j.jpba.2017.05.023
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发表时间：
2017-10-25
影响因子：
3.4
通讯作者：
Ma, Baiping
中科院分区：
文献类型：
--
作者：
Yang, Jie;Zhu, Lingling;Ma, Baiping
超临界流体色谱（SFC）具有良好的分离效果，适用于分离弱极性化合物.呋甾皂苷是一类重要的甾体皂苷，一般具有极性和亲水性两条糖链。呋甾皂苷C-22位的羟基活泼，在适当条件下易与低级醇反应。本研究采用超临界流体技术对亲水性呋甾皂苷进行分离。研究了色谱条件对呋甾皂苷及其C-22位羟基衍生物分离的影响。考察了不同柱极性、不同改性剂、不同添加剂、不同柱温等条件对超临界流体色谱的影响。优化后，在40 ℃下，在Diol柱上22 min内分离混合的10种结构相似的呋甾皂苷。移动的相为CO2（移动的相A）和甲醇（含有0.2%NH3中心点H2O和3%H2O）（移动的相B）。背压维持在11.03MPa的等压。发现SFC可有效分离具有相同糖苷配基但糖链不同的呋甾皂苷。SFC对糖的种类和数量敏感。呋甾皂苷异构体的分离度不理想。以姜芥提取物为原料，H.通过SFC-四极杆飞行时间质谱法对Wright进行分析。提取物中的主要皂苷成分分离良好。因此，超临界流体技术可用于亲水性呋甾皂苷的分离和以甾体皂苷为主的中药成分的分析。(C)2017爱思唯尔B. V.保留所有权利。
Supercritical fluid chromatography (SFC) has good separation efficiency and is suitable for separating weakly polar compounds. Furostanol saponins, as an important kind of steroidal saponins, generally have two sugar chains, which are polar and hydrophilic. The hydroxyl group at the C-22 position of furostanol saponins is active and easily reacts with lower alcohols under appropriate conditions. The separation of hydrophilic furostanol saponins was tested by SFC in this study. The effects of chromatographic conditions on the separation of the mixed furostanol saponins and their hydroxyl derivatives at the C-22 position were studied. The conditions for SFC, which included different column polarity, modifier, additive, and column temperature, were tested. After optimization, the mixed 10 similar structures of furostanol saponins were separated in 22 min on the Diol column at a temperature of 40 degrees C The mobile phase was CO2 (mobile phase A) and methanol (containing 0.2% NH3 center dot H2O and 3% H2O) (mobile phase B). The backpressure was maintained isobarically at 11.03 MPa. SFC was found to be effective in separating the furostanol saponins that shared the same aglycone but varied in sugar chains. SFC was sensitive to the number and type of sugars. The resolution of furostanol saponin isomers was not ideal. The extract of Dioscorea zingiberensis C. H. Wright was profiled by SFC-quadrupole time-of-flight mass spectrometry. The main saponins of the extract were well separated. Therefore, SFC could be used for separating hydrophilic furostanol saponins and analyzing traditional Chinese medicines that mainly contained steroidal saponins. (C) 2017 Elsevier B.V. All rights reserved.