Parthenolide induces proliferation inhibition and apoptosis of pancreatic cancer cells in vitro.
Parthenolide induces proliferation inhibition and apoptosis of pancreatic cancer cells in vitro.
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DOI：
10.1186/1756-9966-29-108
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发表时间：
2010-08-10
期刊：
影响因子：
--
通讯作者：
Huang DS
中科院分区：
文献类型：
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作者：
Liu JW;Cai MX;Xin Y;Wu QS;Ma J;Yang P;Xie HY;Huang DS
探讨孤雌菊对人胰腺癌的抗肿瘤作用。用不同浓度的孤雌菊处理人胰腺癌BxPC-3细胞。MTT法检测细胞活力。应用流式细胞术和DNA片段化分析技术检测孤雌激素处理后细胞凋亡的变化。采用伤口闭合实验和细胞侵袭实验。Western blotting检测Bad、Bcl-2、Bax、caspase-9和pro-caspase-3的表达。四甲基偶氮唑盐（MTT）法表明，独行菊内酯对胰腺癌的生长具有剂量依赖性的抑制作用。流式细胞术和DNA片段化分析证实了这一现象。伤口闭合实验和细胞侵袭实验表明，7.5 μM和15 μM的孤雌菊对BxPC-3细胞有明显的抑制作用。Western Blotting显示Bcl-2和caspase-3蛋白表达下调，Bax和caspase-9蛋白表达上调。治疗后Bad表达无明显改变。银胶菊能抑制胰腺癌细胞的生长、迁移和诱导凋亡。这些发现可能为胰腺癌的治疗提供一种新的方法。
To explore the anti-tumor effects of parthenolide in human pancreatic cancer. BxPC-3 cell, a human pancreatic cancer, was treated with parthenolide at different concentrations. The MTT assay was used to analyze cell viability. Flow cytometry and DNA fragmentation analysis were applied to evaluate apoptosis after parthenolide treatment. The wound closure and cell invasion assay were also employed in the study. Western blotting was used to demonstrate Bad, Bcl-2, Bax, caspase-9 and pro-caspase-3 expression. The MTT assay indicated that the pancreatic cancer growth could be dose-dependently inhibited by parthenoolide. This phenomenon was confirmed by flow cytometry and DNA fragmentation analysis. The wound closure assay and cell invasion assay showed that BxPC-3 cell was significantly suppressed by parthenolide at 7.5 μM and 15 μM. Western Blotting demonstrated the Bcl-2 and pro-caspase-3 were down-regulated while the Bax and caspase-9 were up-regulated. No alteration in Bad expression was found after treatment. The parthenolide can inhibit the cell growth, migration, and induce the apoptosis in human pancreatic cancer. These findings may provide a novel approach for pancreatic cancer treatment.