Identification of a unique membrane-bound molecule on a hemopoietic stem cell line and on multipotent progenitor cells.
Identification of a unique membrane-bound molecule on a hemopoietic stem cell line and on multipotent progenitor cells.
复制标题
鉴定造血干细胞系和多能祖细胞上独特的膜结合分子。
DOI：
10.1073/pnas.92.24.11014
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发表时间：
1995
影响因子：
11.1
通讯作者：
Wong,PM
中科院分区：
文献类型：
--
作者：
Han,XD;Chung,SW;Wong,PM
造血干细胞是一种独特的细胞群，可以分化成多系的造血细胞，并具有长期的再生能力。一些膜结合分子被发现优先存在于这些原始细胞的表面，但不是唯一的。我们在此报告了在造血干细胞BL3表面鉴定出一种独特的105 kda糖蛋白。这种被吸收的抗血清识别的分子不存在于骨髓祖细胞32D和FDC-P1细胞、EL4 T细胞和NIH 3T3成纤维细胞的表面。这种抗血清也可用于阻断BL3细胞的增殖，即使在有丝裂原刺激的脾细胞条件培养基存在的情况下，已知脾细胞条件培养基对BL3细胞具有刺激活性。它还可以抑制体外胎肝细胞衍生的多系集落的发育，但对其他类型的集落没有抑制作用，也可以抑制体内骨髓细胞衍生的集落形成单位脾灶的发育。这些数据表明gp105在造血干细胞分化中起重要作用。
Hemopoietic stem cells are a distinct population of cells that can differentiate into multilineages of hemopoietic cells and have long-term repopulation capability. A few membrane-bound molecules have been found to be preferentially, but not uniquely, present on the surface of these primitive cells. We report here the identification of a unique 105-kDa glycoprotein on the surface of hemopoietic stem cell line BL3. This molecule, recognized by the absorbed antiserum, is not present on the surface of myeloid progenitors 32D and FDC-P1 cells, EL4 T cells, and NIH 3T3 fibroblasts. This antiserum can also be used to block the proliferation of BL3 cells even in the presence of mitogen-stimulated spleen cell conditioned medium, which is known to have a stimulating activity on BL3 cells. It can also inhibit development of in vitro, fetal liver cell-derived multilineage colonies, but not other types of colonies, and of in vivo bone marrow cell-derived colony-forming unit spleen foci. These data suggest that gp105 plays an important role in hemopoietic stem cell differentiation.