Somatic cell cloned transgenic bovine neurons for transplantation in parkinsonian rats.
Somatic cell cloned transgenic bovine neurons for transplantation in parkinsonian rats.
复制标题
体细胞克隆转基因牛神经元用于移植帕金森病大鼠。
DOI：
10.1038/nm0598-569
复制
发表时间：
1998
期刊：
影响因子：
82.9
通讯作者：
Stice,SL
中科院分区：
文献类型：
--
作者：
Zawada,WM;Cibelli,JB;Choi,PK;Clarkson,ED;Golueke,PJ;Witta,SE;Bell,KP;Kane,J;PoncedeLeon,FA;Jerry,DJ;Robl,JM;Freed,CR;Stice,SL
通过将胎儿多巴胺细胞移植到帕金森病患者的壳核中，帕金森病的症状可以得到改善。因为人类捐献组织的供应是有限的和可变的，一种可替代的、可通过基因修改的非人类组织来源将是有价值的。我们已经获得了克隆的转基因牛胚胎，其中42%的胚胎发育超过40天。从受孕后42至50天的克隆胎儿腹侧中脑收集的多巴胺细胞移植到免疫抑制的帕金森病大鼠体内后存活，克隆胚胎和野生型胚胎的细胞改善了运动能力。体细胞克隆可以有效地产生治疗帕金森症的转基因动物组织。
Parkinson's disease symptoms can be improved by transplanting fetal dopamine cells into the putamen of parkinsonian patients. Because the supply of human donor tissue is limited and variable, an alternative and genetically modifiable non-human source of tissue would be valuable. We have generated cloned transgenic bovine embryos, 42% of which developed beyond 40 days. Dopamine cells collected from the ventral mesencephalon of the cloned fetuses 42 to 50 days post-conception survived transplantation into immunosuppressed parkinsonian rats and cells from cloned and wild-type embryos improved motor performance. Somatic cell cloning can efficiently produce transgenic animal tissue for treating parkinsonism.