Retinal nerve fibre layer imaging compared with histological measurements in a human eye
Retinal nerve fibre layer imaging compared with histological measurements in a human eye
复制标题
DOI：
10.1038/sj.eye.6702942
复制
发表时间：
2009-01-01
期刊：
影响因子：
3.9
通讯作者：
Thomas, R.
中科院分区：
文献类型：
--
作者：
Blumenthal, E. Z.;Parikh, R. S.;Thomas, R.
目的比较视网膜神经纤维层（RNFL）厚度值与成像设备获得的RNFL厚度测量值在人眼中的组织学获得的可行性研究。设计一名计划进行眼眶摘除术的患者，他仍然拥有健康功能的眼睛。方法手术前，使用光学相干断层扫描（OCT）和扫描激光偏振仪（SLP）对眼睛进行成像。结果RNFL的厚度与径向子午线上距视盘中心的距离呈负相关。发现组织学、OCT和SLP的视乳头周围RNFL厚度相当。RNFL厚度测量组织学证实了“双峰”模式，峰值在上级和下极。在3.0和3.5mm直径环处，组织学衍生的RNFL厚度（μ m）分别在30-135和25-115之间。相比之下，3.0 mm直径的GDx数据范围在25和100之间，3.4 mm直径的OCT数据范围在40和175之间。定量差异可能部分是由于缩放差异和组织学伪影。在这项研究中证明的组织学分析方法可以潜在地用于验证成像衍生的数据，以及帮助提高我们对青光眼中RNFL丢失的理解。
Purpose A feasibility study comparing retinal nerve fibre layer (RNFL) thickness values obtained with imaging devices against RNFL thickness measurements obtained histologically in a human eye. Design A single patient scheduled for orbital exenteration, who still possessed a healthy functioning eye.Methods Before surgery, the eye was imaged using optical coherence tomography (OCT) and scanning laser polarimetry (SLP). After orbital exenteration, the globe was sectioned, and 100 equidistant RNFL thickness measurements were obtained for each of four concentric rings centred on the optic disc, with diameters of 3.0, 3.5, 4.0, and 4.5mm.Results RNFL thickness was found to be inversely related to the distance from the centre of the optic disc along each radial meridian. Peripapillary RNFL thickness was found comparable for histology, OCT, and SLP. RNFL thickness measured histologically confirmed a 'double hump' pattern, peaking at the superior and inferior poles. Histologically derived RNFL thickness (mu m), at 3.0 and 3.5mm diameter ring ranged between 30-135 and 25-115 respectively. In comparison, the 3.0 mm diameter GDx data ranged between 25 and 100, and the 3.4 mm diameter OCT data between 40 and 175.Conclusions Imaging data appear qualitatively similar when compared to the histologically derived data. Quantitative differences may be partly due to scaling differences and histological artefacts. The histological analysis approach demonstrated in this study can potentially serve to validate imaging-derived data, as well as help improve our understanding of RNFL loss in glaucoma.