The relationship between expressions of the laminin gene and RET gene in Hirschsprung’s disease
The relationship between expressions of the laminin gene and RET gene in Hirschsprung’s disease
复制标题
先天性巨结肠中层粘连蛋白基因和RET基因表达的关系
DOI：
10.1007/s12519-008-0027-5
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发表时间：
2008
影响因子：
8.7
通讯作者：
Yiyan Ruan
中科院分区：
文献类型：
--
作者：
Aiwu Li;Wen;Rong Wang;J. Feng;Yiyan Ruan
研究背景先天性巨结肠（Hirschsprung 'sdisease，HD）的病因尚不清楚，目前主要有两种学说：RET基因突变和肠道微环境改变。本研究进行了阐明HD的原因，通过评估层粘连蛋白（LN），层粘连蛋白基因，RET基因的表达在无神经节段，移行区和正常段的结肠HD患者。MethodsSpecimens的无神经节段，移行区，正常段的结肠HD 27例进行了免疫组织化学染色PV 9000聚合物检测系统。用RS图像系统拍摄照片，用JD 801图像分析系统计算每张图像的染色面积。采用逆转录-聚合酶链反应（RT PCR）检测27例大肠癌中3个节段的层粘连蛋白基因和RET基因的定性表达，并采用α 9900图像分析系统显示其表达差异。采用实时荧光定量PCR方法检测层粘连蛋白基因和RET基因在3个节段中的定量表达，并用SDS软件分析其表达差异。RET基因在无神经节段的表达高于扩张段，从无神经节段到正常段表达逐渐降低，而RET基因的表达则相反，从无神经节段到正常段表达逐渐增加。结论LN在无神经节段的高表达可能导致肠神经细胞的早分化、早成熟和早脱落。结肠壁微环境的改变可能是HD发生的原因。这两个基因表达之间的负相关性可能与HD的发生密切相关。
BackgroundThe cause of Hirschsprung’s disease (HD) remains unclear, but currently there are two theories: the mutation of the RET gene and the change of enteric microenvironment. This study was undertaken to elucidate the cause of HD by assessing the expression of laminin (LN), laminin gene, and the RET gene in the aganglionic segment, transitional zone and normal segment of the colon in patients with HD.MethodsSpecimens of the aganglionic segment, transitional zone, and normal segment of the colon from 27 cases of HD were stained immunohistologically by a PV 9000 polymer detection system. Photos were taken by the RS image system, and the staining area of each image was calculated by a JD 801 image analysis system. The qualitative expressions of the laminin gene and RET gene of these three segments in the 27 cases were detected by reverse transcription-polymerase chain reaction (RTPCR), and the difference of the expressions was shown by the alpha 9900 image analysis system. The quantitative expressions of the laminin gene and RET gene in the three segments were detected by real-time quantitative PCR, and the difference of the expression was shown by SDS software.ResultsThe laminin and laminin gene were expressed in all the three segments. The expression was higher in the aganglionic segment than in the dilated segment, and the expression decreased stepwisely from the aganglionic segment to the normal segment, while the expression of the RET gene was opposite, showing an increased segmenting from the aganglionic segment to the normal segment. The correlation between the expressions of the two genes was negatively correlated.ConclusionsThe highly increased expression of LN in the aganglionic segment may cause early differentiation, early maturation and premature ecesis of enteric nervous cells. The change of the microenvironment of colon wall may be the cause of HD. The negative correlation between the expression of the two genes may be closely related to the occurrence of HD.
ls/ls 小鼠无神经节肠中层粘连蛋白-1 和胶原蛋白 (IV) 亚基的表达增加，但 c-ret -/- 小鼠的无神经节肠中层粘连蛋白-1 和胶原蛋白 (IV) 亚基的表达增加。
DOI：
10.1006/dbio.1996.0234
发表时间：
1996
期刊：
Developmental biology.
影响因子：
--
作者：
Rothman,TP;Chen,J;Howard,MJ;Costantini,F;Schuchardt,A;Pachnis,V;Gershon,MD
通讯作者：
Gershon,MD