Lens placode planar cell polarity is dependent on Cdc42-mediated junctional contraction inhibition.
Lens placode planar cell polarity is dependent on Cdc42-mediated junctional contraction inhibition.
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DOI：
10.1016/j.ydbio.2016.02.016
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发表时间：
2016-04-01
影响因子：
2.7
通讯作者：
Plageman TF Jr
中科院分区：
文献类型：
--
作者：
Muccioli M;Qaisi D;Herman K;Plageman TF Jr
晶状体的发育始于晶状体基板的形成，这是一种上皮结构，增厚并随后向内弯曲，这一过程称为内翻。在许多胚胎结构的发育过程中可以观察到内陷，但在大多数情况下，驱动这一过程的形态发生事件的频谱尚不完全清楚。在进行上皮重组的胚胎组织中常见的一个特征是平面极性，这是细胞沿着上皮平面沿特定方向几何和/或分子定向的一种特性。众所周知，平面极性可以驱动几种上皮结构的形态发生，但其在内陷事件中的作用尚不清楚。我们发现，在内翻的开始，晶状体基板的细胞成为几何平面极化，使它们朝向晶状体基板的中心点。进一步的研究表明，这是由于径向取向结的收缩和周向取向结的伸长。径向连接有升高的肌动球蛋白定位，其收缩依赖于f -肌动蛋白和rho激酶结合蛋白Shroom3。环结的伸长取决于Cdc42，一种已知通过par复合物调节极性的Rho-GTPase。我们发现Cdc42和par复合物的成员抑制shroom3诱导的收缩性，并通过抑制连接收缩促进各向异性的基板细胞几何形状。我们假设晶状体位的内陷需要仔细协调这些相反的过程，这是由连接蛋白的平面极化介导的。
Development of the ocular lens commences with the formation of the lens placode, an epithelial structure that thickens and subsequently bends inward in a process called invagination. Invagination is observed during the development of many embryonic structures, but the spectrum of morphogenetic events driving this process are, in most cases, not fully understood. A characteristic commonly found in embryonic tissues undergoing epithelial reorganization is planar polarity, a property where cells are geometrically and/or molecularly orientated in a specific direction along the plane of an epithelium. Planar polarity is known to drive the morphogenesis of several epithelial structures, however its role during invagination events is less clear. We have found that at the onset of invagination, cells of the lens placode become geometrically planar polarized such that they are orientated toward a central point in the lens placode. Further investigation revealed that this is due to contraction of radially orientated junctions and the elongation of those circumferentially orientated. Radial junctions have an elevated localization of actomyosin and their contraction is dependent on the F-actin and Rho-kinase binding protein, Shroom3. Elongation of circumferential junctions is dependent upon Cdc42, a Rho-GTPase known to regulate polarity via the Par-complex. We determined that Cdc42 and members of the Par-complex inhibit Shroom3-induced contractility and promote anisotropic placode cell geometry through inhibition of junctional contraction. We postulate that invagination of the lens placode requires careful orchestration of these opposing processes which are mediated by the planar polarization of junctional proteins.
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影响因子：
21.3
作者：
Ishiuchi, Takashi;Takeichi, Masatoshi
通讯作者：
Takeichi, Masatoshi
石墨烯单层中屈曲驱动的皱纹的各向异性生长
影响因子：
3.5
作者：
Liu, XiaoYi;Wang, FengChao;Wu, HengAn
通讯作者：
Wu, HengAn
DOI：
10.1073/pnas.0603533103
发表时间：
2006-10-31
影响因子：
11.1
作者：
Chen, Lei;Liao, Guanghong;Zheng, Yi
通讯作者：
Zheng, Yi
影响因子：
4
作者：
Hildebrand, JD
通讯作者：
Hildebrand, JD
影响因子：
4.3
作者：
Murdoch JN;Damrau C;Paudyal A;Bogani D;Wells S;Greene ND;Stanier P;Copp AJ
通讯作者：
Copp AJ