Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
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DOI：
10.1038/nbt.3737
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发表时间：
2017-01
影响因子：
46.9
通讯作者：
Zhang F
中科院分区：
文献类型：
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作者：
Zetsche B;Heidenreich M;Mohanraju P;Fedorova I;Kneppers J;DeGennaro EM;Winblad N;Choudhury SR;Abudayyeh OO;Gootenberg JS;Wu WY;Scott DA;Severinov K;van der Oost J;Zhang F
用Cas9靶向多个基因组基因座受到对多个或大表达构建体的需要的限制。在这里，我们表明Cpf1处理自身CRISPR RNA（crRNA）的能力可用于简化多重基因组编辑。使用一个定制的CRISPR阵列，我们可以同时编辑哺乳动物细胞中的四个基因和小鼠大脑中的三个基因。
Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.