Fabrication of a Fibrous Metal-Organic Framework and Simultaneous Immobilization of Enzymes
Fabrication of a Fibrous Metal-Organic Framework and Simultaneous Immobilization of Enzymes
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纤维状金属有机框架的制备和酶的同时固定化
DOI：
10.1021/acsomega.0c00868
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发表时间：
2020
期刊：
影响因子：
4.1
通讯作者：
Feng Wei
中科院分区：
文献类型：
--
作者：
Jiang Zhengfeng;Chen Yao;Xing Menghan;Ji Peijun;Feng Wei
通过将La(III)与1，3，5-苯三甲酸配位，在水溶液中合成了纳米棒状LaMOF金属-有机骨架。将纳米棒状LaMOF在d-氨基酸氧化物酶溶液中裂解，制得纤维状LaMOF，并对酶进行固定化。结合扫描电子显微镜、扫描电子显微镜、X射线光电子能谱和傅立叶变换红外光谱分析，阐明了纤维状LaMOF的形成机理和明显的界面现象。纤维状LaMOF的制备和酶的同时固定化是在室温的水溶液中进行的，不使用任何有机溶剂。这是一个清洁、省时省力的过程。这项工作为纤维状MOF的制备提供了一种独特而干净的方法。潜在地，这种对环境无害的方法可以扩展到固定化其他酶。
A nanorod-like lanthanum metal–organic framework (LaMOF) was synthesized in aqueous solution by coordinating La(III) to the ligand 1,3,5-benzenetricarboxylic acid. The fibrous LaMOF was fabricated by splitting the nanorod-like LaMOF in a solution ofd-amino acid oxidase, and the enzyme was immobilized simultaneously. Based on SEM and TEM images, STEM mapping, and spectra of XPS and FTIR, the mechanism of formation of the fibrous LaMOF and the distinct interfacial phenomena have been elucidated. The fabrication of the fibrous LaMOF and simultaneous immobilization of the enzyme were carried out in aqueous solutions at room temperature, without using any organic solvent. It is a clean and time- and energy-effective process. This work presents a distinct and clean methodology for the fabrication of the fibrous MOF. Potentially, the environmentally benign methodology can be extended to immobilize other enzymes.