A polysaccharide found in Dendrobium nobile Lindl stimulates calcium signaling pathway and enhances tobacco defense against TMV
A polysaccharide found in Dendrobium nobile Lindl stimulates calcium signaling pathway and enhances tobacco defense against TMV
复制标题
金钗石斛 Lindl 中发现的多糖可刺激钙信号通路并增强烟草对 TMV 的防御
DOI：
10.1016/j.ijbiomac.2019.06.179
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发表时间：
2019-09-15
影响因子：
8.2
通讯作者：
Song, Baoan
中科院分区：
文献类型：
--
作者：
Li, Zhurui;Shi, Jing;Song, Baoan
A neutral polysaccharide separated from Dendrobium nobile Lindl was designated as DNPE6(4). It was structurally characterized using a combination of spectral and chemical analysis. Its average molecule weight was 99.2 kDa. The monosaccharide composition was Araf, Glcp, Galp, and Manp in a molar ratio of 2.5:0.9:03:0.8. Their linkage types were -> 1)-L-Araf-(3 ->, -> 1)-D-Galp-(4 ->, -> 1)-D-Manp-(3 ->, -> 1)-D-Galp-(6 ->, -> 1)-D-Manp-(3,6 ->, and T-D-Manp. The polysaccharide was found to have anti-TMV and anti-CMV activities for the first time in vivo. Notably, DNPE6(4) exhibited excellent protective activity against TMV. Furthermore, several proteins related to calcium signaling pathway and pathogen related proteins were up-regulated, and we also found expression levels of EDS1, ICS1, and PR1 involved in SA pathway up-regulated after DNPE6(4) treatment. In addition, some defense enzymes increased in the same condition. All these findings revealed DNPE6(4) was an elicitor to stimulate calcium signaling pathway to enhance the tobacco defense against TMV. This study therefore revealed that DNPE6(4) was a promising antiviral agent for future study. (C) 2019 Elsevier B.V. All rights reserved.
