The fission yeast spore is coated by a proteinaceous surface layer comprising mainly Isp3.
The fission yeast spore is coated by a proteinaceous surface layer comprising mainly Isp3.
复制标题
裂殖酵母孢子被主要包含Isp3的蛋白质表面层覆盖。
DOI：
10.1091/mbc.e13-12-0731
复制
发表时间：
2014-05
影响因子：
3.3
通讯作者：
Nakamura T
中科院分区：
文献类型：
--
作者：
Fukunishi K;Miyakubi K;Hatanaka M;Otsuru N;Hirata A;Shimoda C;Nakamura T
裂殖酵母孢子的最外层主要由lsp 3蛋白组成。isp 3缺失的孢子显示出对热、消化酶和乙醇的耐受性降低。因此，Isp 3构成孢子衣，从而赋予对各种环境胁迫的抗性。孢子是一种休眠细胞，能抵抗各种环境压力。与营养细胞壁相比，孢子壁具有赋予孢子抗性的更广泛的结构。在裂殖酵母裂殖酵母中，多糖葡聚糖和壳聚糖是孢子壁的主要成分;然而，孢子表面的结构仍然未知。我们鉴定了孢子外壳蛋白Isp 3/Meu 4。isp 3破坏剂是可行的，并执行减数分裂核分裂的效率与野生型，但isp 3芽孢杆菌表现出耐热性下降，消化酶，和乙醇。电子显微镜观察表明，野生型孢子壁的最外层区域形成了一个电子致密层。在isp 3孢子中未观察到该层。此外，Isp 3是丰富的检测在这一层的免疫电镜。因此，Isp 3构成孢子衣，从而赋予对各种环境胁迫的抗性。
The outermost layer of fission yeast spore is mainly composed of the lsp3 protein. Isp3-deleted spores show decreased tolerance to heat, digestive enzymes, and ethanol. Thus Isp3 constitutes the spore coat, thereby conferring resistance to various environmental stresses. The spore is a dormant cell that is resistant to various environmental stresses. As compared with the vegetative cell wall, the spore wall has a more extensive structure that confers resistance on spores. In the fission yeast Schizosaccharomyces pombe, the polysaccharides glucan and chitosan are major components of the spore wall; however, the structure of the spore surface remains unknown. We identify the spore coat protein Isp3/Meu4. The isp3 disruptant is viable and executes meiotic nuclear divisions as efficiently as the wild type, but isp3∆ spores show decreased tolerance to heat, digestive enzymes, and ethanol. Electron microscopy shows that an electron-dense layer is formed at the outermost region of the wild-type spore wall. This layer is not observed in isp3∆ spores. Furthermore, Isp3 is abundantly detected in this layer by immunoelectron microscopy. Thus Isp3 constitutes the spore coat, thereby conferring resistance to various environmental stresses.