Design and Performance of a Multi-Point Scan Confocal Microendoscope.
Design and Performance of a Multi-Point Scan Confocal Microendoscope.
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DOI：
10.3390/photonics1040421
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发表时间：
2014-12
期刊：
影响因子：
2.4
通讯作者：
Gmitro AF
中科院分区：
文献类型：
--
作者：
Risi MD;Makhlouf H;Rouse AR;Tanbakuchi AA;Gmitro AF
共聚焦荧光显微内窥镜通过微创手术提供高分辨率的细胞水平成像，但需要快速扫描才能实现体内实时成像。理想的共聚焦成像性能可以通过点扫描系统获得，但体内生物医学成像所需的扫描速率很难实现。通过与固定共聚焦狭缝孔径一起在一个方向上扫描照明线，可以实现非常高的图像采集速度，但代价是图像质量降低。本文介绍了自定义多点孔径修改线扫描多光谱共聚焦显微内窥镜的设计、实现和实验验证。这种新设计提高了线扫描系统的轴向分辨率，同时保持了高成像速率。此外，与线扫描配置相比，先前报道的模拟预测，多点孔径几何结构大大降低了组织散射对图像质量的影响。实验结果证实了这一预测。
Confocal fluorescence microendoscopy provides high-resolution cellular-level imaging via a minimally invasive procedure, but requires fast scanning to achieve real-time imaging in vivo. Ideal confocal imaging performance is obtained with a point scanning system, but the scan rates required for in vivo biomedical imaging can be difficult to achieve. By scanning a line of illumination in one direction in conjunction with a stationary confocal slit aperture, very high image acquisition speeds can be achieved, but at the cost of a reduction in image quality. Here, the design, implementation, and experimental verification of a custom multi-point aperture modification to a line-scanning multi-spectral confocal microendoscope is presented. This new design improves the axial resolution of a line-scan system while maintaining high imaging rates. In addition, compared to the line-scanning configuration, previously reported simulations predicted that the multi-point aperture geometry greatly reduces the effects of tissue scatter on image quality. Experimental results confirming this prediction are presented.