Characterization by electron spin resonance spectroscopy of reactive oxygen species generated by titanium dioxide and hydrogen peroxide
Characterization by electron spin resonance spectroscopy of reactive oxygen species generated by titanium dioxide and hydrogen peroxide
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DOI：
10.1177/154405910508400213
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发表时间：
2005-02-01
影响因子：
7.6
通讯作者：
Kuse-Barouch, K
中科院分区：
文献类型：
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作者：
Lee, MC;Yoshino, F;Kuse-Barouch, K
活性氧基团(ROS)对钛种植体表面修饰和骨结合的影响尚不清楚。本研究的目的是通过5，5-二甲基-1-吡咯啉-N-氧化物(DMPO)的电子自旋共振(ESR)自旋捕捉来评价二氧化钛(TiO2O2)在过氧化氢存在下产生ROS的能力，并确定由此产生的ROS是否在骨整合中发挥作用。我们证明了二氧化钛和过氧化氢一起产生了羟基自由基(HO)，DMPO-OH自旋加合物的ESR信号的自旋浓度随时间的增加表明了HO。一代。有趣的是，用过氧化氢照射的二氧化钛产生了超氧化物(O-2(.-))，这表现为DMPO-OHO自旋加合物信号的自旋浓度增加，表明O-2(-)。在照射期间(0-5分钟)产生。这些结果表明，从二氧化钛层产生的ROS可能参与为牙种植体与牙槽骨组织的骨整合创造适当的条件。
The influence of reactive oxygen species (ROS) on the surface modification of titanium implants and osseointegration is unclear. The aim of this study was to evaluate the ability of titanium dioxide (TiO2) to generate ROS in the presence of H2O2 and to determine whether any ROS thus generated play a role in osseointegration, as measured by electron spin resonance (ESR) spin-trapping with 5,5-dimethyl-1-pyrolline-N-oxide (DMPO). We demonstrate that TiO2 together with H2O2 generated hydroxyl radicals (HO.), as shown by a time-dependent increase in the spin concentration of the ESR signal for the DMPO-OH spin adduct, indicating HO. generation. Interestingly, irradiated TiO2 with H2O2 generated the superoxide (O-2(.-)), as shown by an increase in the spin concentration of the signal for the DMPO-OOH spin adduct, indicating O-2(-). generation during the period of irradiation (0-5 min). These results suggest that ROS generated from the TiO2 layer may be involved in creating appropriate conditions for the osseointegration of dental implants into alveolar bone tissues.