A novel bio‐active adhesive monomer induces odontoblast differentiation: a comparative study
A novel bio‐active adhesive monomer induces odontoblast differentiation: a comparative study
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一种新型生物活性粘合剂单体诱导成牙本质细胞分化：比较研究
DOI：
10.1111/iej.13365
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发表时间：
2020
影响因子：
5
通讯作者：
Saito T.
中科院分区：
文献类型：
--
作者：
Qiu Y. J.;Tang J.;Saito T.
目的探讨新型粘附性单体CMET（4-甲基丙烯酰氧乙基偏苯三酸钙盐，4-MET）对成牙本质细胞样细胞增殖、矿化和分化的影响，并与4-MET、氢氧化钙（CH）和三氧化矿物质聚集体（MTA）进行比较。首先将四种供试材料（CMET、4-MET、CH和MTA）的粉末溶于蒸馏水（dH 2 O）中，然后用DMEM稀释以获得最终浓度。溶剂（dH 2 O）用作对照。使用CCK-8测定法评估细胞活力。采用真实的实时RT-PCR定量牙源性标志物、细胞因子和整合素的mRNA表达。通过碱性磷酸酶（ALP 3）活性和茜素红S染色评价矿化诱导能力。结果CMET-（83 ~ 828 mmol L-1）、CH-和MTA-处理组（低浓度）细胞活力显著高于对照组（P<0. 01）。高浓度的各物质均不同程度地降低活细胞数（P< 0.01）。CMET组牙胚中整合素亚基的表达增加，牙源性标记物的mRNA表达水平最高（P< 0.01）。CH和MTA治疗引起的促炎细胞因子表达的上调显著大于其他组（P< 0.01）。添加CMET后MDPC-23细胞的钙化沉积呈剂量依赖性加速（P< 0.01）;在新鲜制备的CH和MTA处理中也发现矿化增强。此外，CMET在储存8周后表现出一致的矿化诱导。结论新型生物活性单体SB 202190对成牙本质样细胞的细胞毒性最低，在适当浓度下可诱导成牙本质样细胞增殖、矿化和分化。该粘合剂单体具有良好的生物相容性，因此在牙本质再生方面具有很大的潜力。
AimTo evaluate thein vitroeffect of the novel adhesive monomer CMET, a calcium salt of 4‐methacryloxyethyl trimellitate (4‐MET), on the proliferation, mineralization and differentiation of odontoblast‐like cells, comparing with 4‐MET, calcium hydroxide (CH) and mineral trioxide aggregate (MTA).MethodologyRat odontoblast‐like MDPC‐23 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 5% foetal bovine serum. The powder of four tested materials (CMET, 4‐MET, CH and MTA) was first dissolved in distilled water (dH2O) and then was diluted by DMEM to yield final concentrations. Solvent (dH2O) was used as a control. Cell viability was assessed using CCK‐8 assay. Real‐time RT‐PCR was used to quantify the mRNA expression of odontogenic markers, cytokines and integrins. Mineralization inducing capacity was evaluated by alkaline phosphatase (ALPase) activity and alizarin red S staining. Statistical analyses were performed using one‐wayanovaandpost hocTukey’s HSD test, with the significance level at 1%.ResultsCell viability was significantly greater in the CMET‐ (83 to 828 mmol L−1), CH‐ and MTA‐treated (low concentrations) groups than that in the control group (P< 0.01). Higher concentrations of each material decreased the viable cells to different extents (P< 0.01). CMET treatment augmented the expression of several integrin subunits and exhibited the highest mRNA expression levels of odontogenic markers among all groups (P< 0.01). CH and MTA treatment caused significantly greater upregulation of pro‐inflammatory cytokines expression than the other groups (P< 0.01). The calcific deposition of MDPC‐23 cells was dose‐dependently accelerated by the addition of CMET (P< 0.01); the enhancement of mineralization was also found in the fresh prepared CH and MTA treatments. Besides, CMET showed consistency in mineralization induction after 8 weeks storage. Exposure to SB202190, a specific p38 mitogen‐activated protein kinases inhibitor, significantly decreased the ALPase activity as well as the mineral deposition which was enhanced by CMET treatment (P< 0.01).ConclusionsThe novel bio‐active monomer had the lowest cytotoxicity among all groups and it induced the proliferation, mineralization and differentiation of odontoblast‐like cells under appropriate concentrations. This adhesive monomer possesses excellent biocompatibility and hence exhibits great potential in dentine regeneration.