Guanidine-dependent mutants of poliovirus: identification of three classes with different growth requirements.
Guanidine-dependent mutants of poliovirus: identification of three classes with different growth requirements.
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DOI：
10.1016/0042-6822(87)90316-3
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发表时间：
1987-03
期刊：
影响因子：
3.7
通讯作者：
S. Pincus;H. Røhl;E. Wimmer
中科院分区：
文献类型：
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作者：
S. Pincus;H. Røhl;E. Wimmer
四个突变体耐高（2.0 mM）胍衍生自突变体耐中间（0.53 mM）水平的这种药物。发现这些突变体中的一个对高胍具有抗性，并且显示出含有先前在这类突变体中观察到的2C内的突变，而缺乏在中间亲本中观察到的突变。其他三个突变体依赖于胍生长，并含有在亲本病毒中观察到的2C突变以及先前在另一个依赖性突变体中观察到的突变。新分离的依赖性突变体的两个先前描述的依赖性突变体的比较显示，可以观察到三个类的依赖性突变体，其不同的最佳生长的要求。我们提出了一个模型，胍与2C的相互作用，解释了三个类的依赖突变体的发生。
Four mutants resistant to high (2.0 mM) guanidine were derived from a mutant resistant to intermediate (0.53 mM) levels of this drug. One of these mutants was found to be resistant to high guanidine and was shown to contain a mutation within 2C seen previously in this class of mutants, while lacking the mutation seen in the intermediate parent. The other three mutants were dependent on guanidine for growth and contained the mutation in 2C seen in the parental virus as well as a mutation seen previously in another dependent mutant. Comparison of the newly isolated dependent mutants to two previously described dependent mutants revealed that three classes of dependent mutants which vary in their requirements for optimal growth can be observed. We present a model for the interaction of guanidine with 2C that explains the occurrence of the three classes of dependent mutants.