JAPANESE MACAQUE MICROSATELLITE PCR PRIMERS FOR PATERNITY TESTING
JAPANESE MACAQUE MICROSATELLITE PCR PRIMERS FOR PATERNITY TESTING
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DOI：
10.1007/bf02381278
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发表时间：
1993-01-01
期刊：
影响因子：
1.7
通讯作者：
TAKENAKA, O
中科院分区：
文献类型：
--
作者：
INOUE, M;TAKENAKA, O
在野生灵长类动物种群中捕获和采血是困难的。因此，我们需要使用从目标动物的毛发或粪便中提取的DNA。聚合酶链反应（PCR）方法，扩增小体积的DNA，提供了一个理想的手段，研究DN的变化在野生种群。以日本猕猴（Macaca fuscata）DNA序列为模板，设计合成了3对能扩增高度多态性（GT/AC）n二核苷酸重复区的PCR引物。其中一对引物在一个圈养的日本猕猴群体中检测到至少7个等位基因。此外，还确定了四只后代的父亲，这些后代的母亲在一群圈养的日本猕猴中死亡。在这种情况下，不能确定父亲的th以前的DNA指纹法的基础上的小卫星DNA的多态性。这些引物进一步测试与一些种类的猕猴，如。grivet猴（Cercopithecus aethiops tantalus）和hamadryas狒狒（Papio hamadryas）。结果表明，这些引物可以检测到每个物种中稳定遗传的多态性区域。
Capture and blood sampling in wild primate populations are difficult. For this reason, we need to use DNA extracted from the hair or feces of target animals. The polymerase chain reaction (PCR) method, which amplifies small volumes of DNA, provides an ideal means for studying DN variations in wild populations. Three sets of PCR primers which amplify highly polymorphic (GT/AC)n dinucleotide repetitive regions were synthesized from DNA sequences of Japanese macaques (Macaca fuscata). One of the primer pairs detected at least seven alleles in one captive Japanese macaque group. Also, the fathers of four offspring whose mothers had died in a captive group of Japanese macaques were identified. In such cases, the father cannot be determined by th previous DNA fingerprinting method based on the polymorphism of minisatellite DNA. These primers were further tested with some species of the Cercopithecidae, eg. grivet monkeys (Cercopithecus aethiops tantalus) and hamadryas baboons (Papio hamadryas). The results obtained suggest th these primers can detect stably inherited polymorphic regions in each species.