Serum keratan sulfate is a promising marker of early articular cartilage breakdown
Serum keratan sulfate is a promising marker of early articular cartilage breakdown
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DOI：
10.1093/rheumatology/kem220
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发表时间：
2007-11-01
期刊：
影响因子：
5.5
通讯作者：
Miyazaki, K.
中科院分区：
文献类型：
--
作者：
Wakitani, S.;Nawata, M.;Miyazaki, K.
目标.目的寻找可作为早期诊断关节软骨退变或损伤的血清学指标。对24名健康志愿者、19名膝关节创伤（KT）患者和31名膝关节骨关节炎（OA）患者进行了评价。KT患者分为损伤时间< 2个月（近期KT）组（n=5）和损伤时间> 2个月（陈旧KT）组（n=14）。通过关节镜或直接观察评估关节软骨损伤。采用酶联免疫吸附法检测血清透明质酸（HA）、软骨蛋白多糖聚集蛋白聚糖转换表位（CS 846）和软骨寡聚蛋白（COMP）的浓度，采用高效液相色谱法检测血清硫酸角质素（KS）和6-硫酸软骨素（C6 S）的浓度。近期KT组血清KS（2095 ± 594 ng/ml）显著高于老年KT组（1373 +/- 418 ng/ml; P=0.021），近期KT组血清COMP（1572 +/-182 ng/ml）呈高于老KT组的趋势（1350 ± 250 ng/ml; P=0.079）。Kellgren和Lawrence（KL）分级为0和1的OA患者的血清KS（1456 ± 334 ng/ml）高于KL Ⅱ、Ⅲ、Ⅳ级OA患者（1248 ± 220 ng/ml; P=0.084）。血清KS浓度与关节软骨损伤程度相关，在损伤早期即明显升高。
Objectives. To find serum markers that may serve as indices for an early diagnosis of degeneration or damage of the articular cartilage.Methods. Twenty-four healthy volunteers, 19 individuals with knee trauma (KT) and 31 with knee osteoarthritis (OA) were evaluated. KT patients were divided into a group (n=5) with an injury < 2 months old (recent KT) and a group (n=14) with that > 2 months old (old KT). Articular cartilage damage was assessed using either arthroscopy or direct observation. Serum concentrations of hyaluronic acid (HA), cartilage proteoglycan aggrecan turnover epitope (CS846) and cartilage oligomeric protein (COMP) were measured using enzyme-linked immunosorbent assay kits and those of keratan sulfate (KS) and chondroitin-6-sulfate (C6S) using high-performance liquid chromatography.Results. Serum KS in the recent KT group (2095 +/- 594 ng/ml) was significantly higher than that in the old KT group (1373 +/- 418 ng/ml; P=0.021), and serum COMP in the recent KT group (1572 +/- 182 ng/ml) showed a tendency that was higher than that in the old KT group (1350 +/- 250 ng/ml; P=0.079).Serum KS in OA patients with Kellgren and Lawrence (KL) grades 0 and 1 (1456 +/- 334 ng/ml) showed a tendency that was higher than that in OA patients with KL grades II, III and IV (1248 +/- 220 ng/ml; P=0.084).Conclusions. The serum concentration of KS correlated with the damage of the articular cartilage and it was significantly increased even at an early stage after the injury.