Purinergic endothelium-dependent and -independent contractions in rat aorta.
Purinergic endothelium-dependent and -independent contractions in rat aorta.
复制标题
大鼠主动脉中嘌呤能内皮依赖性和非依赖性收缩。
DOI：
10.1161/01.hyp.22.4.577
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Vanhoutte,PM
中科院分区：
文献类型：
--
作者：
Mombouli,JV;Vanhoutte,PM
在自发性高血压大鼠(SHR)、血压正常的Wistar-京都(WKY)大鼠和Wistar大鼠的主动脉上，观察了内皮衍生收缩因子对腺嘌呤核苷酸的舒缩和收缩作用。在苯肾上腺素的收缩过程中，与WKY血管内皮相比，SHR对ATP的松弛作用明显减弱。在用NG-硝基-L-精氨酸(抑制一氧化氮合酶)处理的血管中，血管内皮细胞显著增强三磷酸腺苷引起的收缩；这种增强作用在自发性高血压大鼠比WKY大动脉更明显。环氧合酶抑制剂吲哚美辛和血栓烷/前列腺素受体拮抗剂SQ 29,458可显著增强WKY大鼠的最大舒张性，消除自发性高血压大鼠的舒张性损害，并阻止血管内皮对ATP引起的收缩的增强作用。在老年动物(10~12月龄)，经消炎痛处理的SHR和WKY动脉对三磷酸腺苷的内皮依赖性浓度-松弛曲线和浓度-收缩曲线(有NG-硝基-L-精氨酸存在)是可叠加的。这些制剂对ADP的内皮依赖性浓度松弛和收缩曲线也重叠。与其他品系相比，Wistar大鼠对ATP或ADP的内皮依赖性松弛幅度明显减小，内皮依赖性收缩幅度更小。结果表明，腺嘌呤核苷酸刺激内皮衍生的舒缩因子的产生。虽然大鼠主动脉释放内皮衍生的舒缩因子的能力没有明显的年龄相关性改变，但衰老增强了WKY大鼠的内皮衍生收缩因子的活性。
The role of endothelium-derived contracting factor or factors in modulating relaxations and contractions to adenine nucleotides was examined in aortas from spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) and Wistar rats. During contractions to phenylephrine, the relaxations to ATP were impaired significantly in SHR compared with WKY aortas with endothelium. In rings treated with NG-nitro-L-arginine (to inhibit nitric oxide synthase), the endothelium significantly augmented contractions evoked by ATP; this enhancement was greater in SHR compared with WKY aortas. Indomethacin (inhibitor of cyclooxygenase) and SQ 29,458 (antagonist of thromboxane/prostaglandin endoperoxide receptors) but not dazoxiben (inhibitor of thromboxane synthase) significantly augmented the maximal relaxation in WKY rats, abolished the impairment of the relaxation in SHR, and prevented the potentiation by the endothelium of the contractions evoked by ATP. In older animals (10 to 12 months old), the endothelium-dependent concentration-relaxation curves to ATP in SHR and WKY aortas treated with indomethacin were superimposable, as were the concentration-contraction curves (with NG-nitro-L-arginine present). Endothelium-dependent concentration-relaxation and -contraction curves to ADP obtained in these preparations overlapped also. In Wistar rats, the magnitude of the endothelium-dependent relaxations to either ATP or ADP were significantly smaller compared with the other strains, and the endothelium-dependent contractions were even smaller. Results show that adenine nucleotides stimulate the production of both endothelium-derived relaxing and contracting factors. Although there is no obvious age-related alteration in the capacity of aortas to release endothelium-derived relaxing factor, aging enhances endothelium-derived contracting factor activity in WKY rats.