iPathology cockpit diagnostic station: validation according to College of American Pathologists Pathology and Laboratory Quality Center recommendation at the Hospital Trust and University of Verona.
iPathology cockpit diagnostic station: validation according to College of American Pathologists Pathology and Laboratory Quality Center recommendation at the Hospital Trust and University of Verona.
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DOI：
10.1186/1746-1596-9-s1-s12
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发表时间：
2014
影响因子：
2.6
通讯作者：
Eccher A
中科院分区：
文献类型：
--
作者：
Brunelli M;Beccari S;Colombari R;Gobbo S;Giobelli L;Pellegrini A;Chilosi M;Lunardi M;Martignoni G;Scarpa A;Eccher A
数字化全载玻片图像的验证对于确保诊断性能至少与载玻片和光学显微镜相当至关重要。美国病理学家学院病理学和实验室质量中心最近制定了内部数字病理学系统验证的建议。根据这些指南，我们试图通过一项试点研究，使用iPad和数字控制屏幕辅助工作站，验证数字方法进行常规诊断的性能。自2014年1月起，使用ScanScope Digital Slides Scanner（Aperio，Vista，CA）扫描61张组织病理学切片。两名独立的病理学家在上传iPad的Citrix接收器后，使用位于通过本地网络连接的不同卫生机构（AOUI Verona）的两台计算机设备（ImageScope查看软件）和使用iPad平板电脑（Aperio，Vista，CA）的远程图像服务器，在屏幕前对虚拟载玻片进行诊断。基于主观感知（高与差）对电子健康驾驶舱企业系统的图像特征和工作流程相关的质量指标进行评分。每隔两周对图像进行重新评估。10例肝癌、10例肾癌、10例胃癌和10例前列腺活检组织：腺癌，5例皮肤切除活检组织：黑色素瘤，5例淋巴结：淋巴瘤。6种免疫染色和5种特殊染色可用于内部和互联网远程查看。每个载玻片的平均扫描时间为2分54秒（标准差为2分34秒）。每个载玻片存储的MB范围为256至680（平均390）。对载玻片的依赖性、图像质量（分辨率和色彩保真度）、载玻片导航时间、地理位置偏远的同时观察者被认为是高性能评分。组织载玻片与荧光屏上进行的诊断之间的并排比较非常好，显示出几乎完美的一致性（0.81，kappa指数）。我们在驾驶舱企业数字系统或iPad平板电脑中验证了我们的机构数字病理学系统，用于常规诊断。电脑屏幕更适合诊断iPad扫描的载玻片。对于紧急请求，可以使用iPad。法律的方面必须很快面对，以允许以不损害患者护理的方式临床使用该技术。
Validation of digital whole slide images is crucial to ensure that diagnostic performance is at least equivalent to that of glass slides and light microscopy. The College of American Pathologists Pathology and Laboratory Quality Center recently developed recommendations for internal digital pathology system validation. Following these guidelines we sought to validate the performance of a digital approach for routine diagnosis by using an iPad and digital control widescreen-assisted workstation through a pilot study. From January 2014, 61 histopathological slides were scanned by ScanScope Digital Slides Scanner (Aperio, Vista, CA). Two independent pathologists performed diagnosis on virtual slides in front of a widescreen by using two computer devices (ImageScope viewing software) located to different Health Institutions (AOUI Verona) connected by local network and a remote image server using an iPad tablet (Aperio, Vista, CA), after uploading the Citrix receiver for iPad. Quality indicators related to image characters and work-flow of the e-health cockpit enterprise system were scored based on subjective (high vs poor) perception. The images were re-evaluated two weeks apart. The whole glass slides encountered 10 liver: hepatocarcinoma, 10 renal carcinoma, 10 gastric carcinoma and 10 prostate biopsies: adenocarcinoma, 5 excisional skin biopsies: melanoma, 5 lymph-nodes: lymphoma. 6 immuno- and 5 special stains were available for intra- and internet remote viewing. Scan times averaged two minutes and 54 seconds per slide (standard deviation 2 minutes 34 seconds). Megabytes ranged from 256 to 680 (mean 390) per slide storage. Reliance on glass slide, image quality (resolution and color fidelity), slide navigation time, simultaneous viewers in geographically remote locations were considered of high performance score. Side by side comparisons between diagnosis performed on tissue glass slides versus widescreen were excellent showing an almost perfect concordance (0.81, kappa index). We validated our institutional digital pathology system for routine diagnostic facing with whole slide images in a cockpit enterprise digital system or iPad tablet. Computer widescreens are better for diagnosing scanned glass slide that iPad. For urgent requests, iPad may be used. Legal aspects have to be soon faced with to permit the clinical use of this technology in a manner that does not compromise patient care.