RNA-seq
RNA-seq
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DOI：
10.1201/b16589-2
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发表时间：
2020-11
期刊：
影响因子：
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通讯作者：
E. Curry
中科院分区：
文献类型：
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作者：
E. Curry
本文报道了3例正常子宫内膜、3例非典型子宫内膜增生症和3例子宫内膜样癌的单细胞基因表达数据。他们报告了这些样本的细胞结构，确定了AEH和子宫内膜样子宫内膜癌样本中增加的上皮细胞。他们利用这些数据证实子宫内膜样癌起源于上皮细胞，并假定它起源于未纤毛的腺上皮细胞。他们发现Lcn2和SAA1/2基因的结合可能是肿瘤发生的标志。他们用免疫荧光证实了这两个基因蛋白表达的增加。他们还进一步分析了微环境中其他细胞的基因表达，包括间质细胞和免疫细胞，发现病理之间只有微小的差异，并表明子宫内膜间质细胞可能通过基因表达的变化支持类器官生长。
This paper reports on single cell gene expression data generated from 3 subjects of normal endometrium, 3 subject with atypical endometrial hyperplasia and 3 subjects with epithelial endometrioid endometrial cancer. They report on the cellular architecture of these samples, identifying increased epithelial cells in AEH and endometrioid endometrial cancer samples. They use this data to confirm endometrioid carcinomas are derived from epithelial cells and postulate it is derived from unciliated glandular epithelial cells. They identify genes LCN2 and SAA1/2 in combination may be markers for tumorigenesis. They confirm increased protein expression of these two genes with immunofluorescence. They also go on to analysis gene expression in other cells of the microenvironment including stromal cells and immune cells, finding only minimal differences between pathologies and showing the endometrial stromal cells may support organoid growth through changes in gene expression.