A computer-controlled variable light attenuator for protection and autoranging of a laser-induced fluorescence detector for capillary zone electrophoresis

A computer-controlled variable light attenuator for protection and autoranging of a laser-induced fluorescence detector for capillary zone electrophoresis
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DOI:
10.1002/elps.1150191232
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发表时间:
1998-09-01
期刊:
影响因子:
2.9
通讯作者:
Mort, AJ
Mort, AJ
中科院分区:
生物学3区
文献类型:
--
作者:
Merz, JM;Mort, AJ

文献摘要

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光电探测器有一个有限的范围,在这个范围内,它们可以测量任何特定环境的光强度。通过使用由计算机控制的液晶可变光衰减器,可以将到达探测器的光的强度保持在该范围内,该液晶可变光衰减器连续检查到达探测器的光量,并将衰减调节到适当的水平。使用这样的系统,我们已经构建了一个基于增强型电荷耦合器件(ICCD)相机的探测器,其动态范围超过六个数量级,永远不会暴露在损坏或饱和的光级中。
Photodetectors have a limited range over which they can measure light intensities for any particular setting. The intensity of light reaching the detector can be kept within this range by using a liquid crystal variable light attenuator controlled by a computer that continuously checks the amount of light reaching the detector and adjusts the attenuation to an appropriate level. Using such a system we have constructed an intensified charge-coupled device (ICCD) camera-based detector with a dynamic range of over six orders of magnitude which is never exposed to damaging or saturating light levels.